嗜热古菌火球菌K1中天冬氨酰-tRNA合成酶对苏氨酸tRNA的分子识别

Molecular recognition of threonine tRNA by threonyl-tRNA synthetase from an extreme thermophilic archaeon, Aeropyrum pernix K1.

作者信息

Nagaoka Yoshiyuki, Yokozawa Junji, Umehara Takuya, Iwaki Jun, Okamoto Koji, Kawarabayasi Yutaka, Koyama Yoshinori, Sako Yoshihiko, Wakagi Takayoshi, Kuno Atsushi, Hasegawa Tsunemi

机构信息

Department of Material and Biological Chemistry, Faculty of Science, Yamagata University, Yamagata 990-8560, Japan.

出版信息

Nucleic Acids Res Suppl. 2002(2):81-2. doi: 10.1093/nass/2.1.81.

DOI:10.1093/nass/2.1.81

PMID:12903115

Abstract

To investigate the recognition sites of tRNA(Thr) for threonyl-tRNA synthetase (ThrRS) from an extreme thermophilic and aerobic archaeon, Aeropyrum pernix K1, threonylation experiments using various in vitro mutant transcripts of tRNA(Thr) were examined. The results indicated that A. pernix ThrRS did recognize the first three base pairs of acceptor stem in addition to the second and the third letters of anticodon of tRNA(Thr), in spite of its N-terminal truncated unique structure. Discriminator base was not involved in recognition by A. pernix ThRS. These determinants were confirmed by the identity switching experiments from the in vitro mutants of A. pernix tRNA(Pro) and tRNA(Asn).

摘要

为了研究来自极端嗜热需氧古菌——火球菌K1（Aeropyrum pernix K1）的苏氨酰-tRNA合成酶（ThrRS）对tRNA（Thr）的识别位点，我们检测了使用tRNA（Thr）各种体外突变转录本进行的苏氨酰化实验。结果表明，尽管火球菌ThrRS的N端具有截短的独特结构，但它除了识别tRNA（Thr）反密码子的第二个和第三个碱基外，还确实识别受体茎的前三个碱基对。鉴别碱基不参与火球菌ThrRS的识别。通过火球菌tRNA（Pro）和tRNA（Asn）体外突变体的身份转换实验证实了这些决定因素。