双（叔丁基）-1-甲基芘基硅氯作为核苷5'-羟基保护基团及寡核苷酸合成中荧光纯化手柄的合成与应用。
Synthesis and application of bis (tert-butyl)-1-methyl pyrenyl-silyl chloride as a 5'-hydroxyl protecting group for nucleosides and as a fluorescent purification handle in oligonucleotide synthesis.
作者信息
Tripathi Snehlata, Misra Krishna, Sanghvi Yogesh S
机构信息
Nucleic Acids Research Laboratory, Department of Chemistry, University of Allahabad, Allahabad-211002, India.
出版信息
Nucleic Acids Res Suppl. 2002(2):117-8. doi: 10.1093/nass/2.1.117.
PMID:12903133
Abstract
A fluorescently labelled monomer viz. 5'-O-1, 1-bis-(tert-butyl)-1'-methyl-pyrenyl-silyl-thymidine-3'-phosphoramidite was synthesized. Different sequences were synthesized and were labelled using it and their fluorescence studies were carried out.
摘要
合成了一种荧光标记的单体，即5'-O-1,1-双（叔丁基）-1'-甲基-芘基-硅烷基-胸腺嘧啶-3'-亚磷酰胺。合成了不同的序列，并用其进行标记，然后开展了它们的荧光研究。
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