Moriguchi Tomohisa, Hattori Masayoshi, Sekiguchi Tohru, Ichimura Mayumi, Kato Takahiko, Shinozuka Kazuo
Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Tenjincho 1-5-1, Kiryu, Gunma, 376-8515, Japan.
Nucleic Acids Symp Ser (Oxf). 2008(52):255-6. doi: 10.1093/nass/nrn129.
Preparation and properties of the novel fluorescently labelled oligonucleotides, containing the silylated pyrene derivatives are reported. The silylated pyrene derivatives were introduced into both 5'-terminus and C-5 position of the deoxyuridine derivatives. The fluorescent spectra indicated the possibility of the discrimination of the duplex formation by the simple denaturation.
报道了含有硅烷化芘衍生物的新型荧光标记寡核苷酸的制备及其性质。硅烷化芘衍生物被引入到脱氧尿苷衍生物的5'-末端和C-5位。荧光光谱表明通过简单变性来区分双链体形成的可能性。
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