新型碱基识别荧光（BDF）核苷的合成与性质

Synthesis and properties of novel base-discriminating fluorescent (BDF) nucleosides.

作者信息

Saito Yoshio, Hanawa Kazuo, Hayashi Keigo, Motegi Kaori, Okaoto Akimitsu, Saito Isao

机构信息

NEWCAT Institue, School of Engineering, Nihon University and SORST, Japan Science and Tecnology Agency, Tamura, Koriyama, 963-8642, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2005(49):153-4. doi: 10.1093/nass/49.1.153.

DOI:10.1093/nass/49.1.153

PMID:17150679

Abstract

We designed a new type of pyrene-labeled base-discrimination fluorescent (BDF) nucleosides (Py)U, (Py)C, (8Py)A and (MePy)dA, which emitted strong fluorescence only when the bases opposite the BDF base are A, G, T and C, respectively. The DNA probes containing four different BDF bases enable us to distinguish single base alterations by simply mixing with a sample solution of target DNA.

摘要

我们设计了一种新型的芘标记的碱基识别荧光（BDF）核苷（Py）U、（Py）C、（8Py）A和（MePy）dA，它们仅在与BDF碱基相对的碱基分别为A、G、T和C时才发出强烈荧光。含有四种不同BDF碱基的DNA探针使我们能够通过与靶DNA样品溶液简单混合来区分单碱基改变。