Tonomoto Riko, Sonoda Hiroyuki, Suzuki Katsunori, Yoshida Kazuo
Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima 739-8526, Japan.
Nucleic Acids Res Suppl. 2002(2):233-4. doi: 10.1093/nass/2.1.233.
To establish in vitro trans-kingdom conjugation, we developed a novel direct cell PCR by using toluenized cells which permeate enzymes, primers and substrates. The cells was shown to be successful recipients in conjugation.
为了建立体外跨菌属接合，我们利用经甲苯处理的细胞（其可渗透酶、引物和底物）开发了一种新型的直接细胞PCR。结果表明这些细胞是接合过程中成功的受体。
