Nakano Toshiaki, Terato Hiroaki, Yoshioka Yoshihiro, Ohyama Yoshihiko, Kubo Kihei, Ide Hiroshi
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
Nucleic Acids Res Suppl. 2002(2):239-40. doi: 10.1093/nass/2.1.239.
When DNA is exposed to NO or HNO2, oxanine (Oxa) is formed as a major guanine lesion. For highly sensitive detection of Oxa using ARP, a probe molecule for DNA damage detection, the reactivity of ARP to Oxa was examined. Oxa site-specifically embedded in an oligonucleotide reacted with ARP but it took relatively long time (ca. 24 h) to completely convert Oxa to an ARP-labeled form.
当DNA暴露于一氧化氮（NO）或亚硝酸（HNO2）时，氧嘌呤（Oxa）作为主要的鸟嘌呤损伤产物形成。为了使用用于DNA损伤检测的探针分子ARP对Oxa进行高灵敏度检测，研究了ARP对Oxa的反应性。特定位点嵌入寡核苷酸中的Oxa与ARP发生反应，但将Oxa完全转化为ARP标记形式需要相对较长的时间（约24小时）。
