Kurisu S, Miya T, Terato H, Masaoka A, Ohyama Y, Kubo K, Ide H
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
Nucleic Acids Res Suppl. 2001(1):45-6. doi: 10.1093/nass/1.1.45.
DNA is constantly damaged by endogenous and environmental agents. Abasic sites representing a major class of DNA damage can be quantitated by an ELISA-like assay using an aldehyde reactive probe (ARP). It is shown that oxidative pyrimidine lesions can be also quantitated by the ARP assay in combination with the treatment with endonuclease III.
DNA 不断受到内源性和环境因素的损伤。无碱基位点是一类主要的 DNA 损伤，可通过使用醛反应探针（ARP）的类似酶联免疫吸附测定（ELISA）的方法进行定量。结果表明，氧化嘧啶损伤也可通过 ARP 测定结合核酸内切酶 III 处理进行定量。
