DNA双链形成的新热力学表征及转变机制
New thermodynamic characterization and transition mechanism of DNA duplex formation.
作者信息
Wu P, Sugimoto N
机构信息
High Technology Research Center, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
出版信息
Nucleic Acids Symp Ser. 2000(44):15-6. doi: 10.1093/nass/44.1.15.
PMID:12903246
Abstract
A new transition mechanism of DNA duplex association was proposed and a segregated transition model (STM) was further derived. The experimental results in various molar ratios showed that the duplex association transition is imperfect and the thermodynamic properties and self-transition behavior of single strands exert a significant influence on DNA duplex formation.
摘要
提出了一种新的DNA双链体缔合转变机制，并进一步推导了一种分离转变模型（STM）。不同摩尔比下的实验结果表明，双链体缔合转变是不完全的，单链的热力学性质和自转变行为对DNA双链体形成有显著影响。
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