Fujiwara T, Sugiyama H, Hirao I, Yokoyama S
Yokoyama CytoLogic Project, ERATO, JST, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Nucleic Acids Symp Ser. 2000(44):43-4. doi: 10.1093/nass/44.1.43.
Unnatural bases specifically pairing with pyridin-2-one, 2-amino-6-(2-thienyl) purine and 2-amino-6-(2-furanyl)purine, were newly designed to replace 2-amino-6-(N,N-dimethylamino)purine. It was expected that these novel purine analogues, as compared with 2-amino-6-(N,N-dimethylamino)purine, might reduce the interference in the stacking interactions with the neighboring bases in a duplex and improve the efficiency of the enzymatic incorporation of the nucleoside triphosphate of pyridin-2-one opposite these unnatural bases. The syntheses of these nucleoside derivatives and the DNA fragments were examined.
