Sola L, Gornung E, Naoi H, Gunji R, Sato C, Kawamura K, Arai R, Ueda T
Department of Animal and Human Biology, University of Rome I La Sapienza, via A. Borelli, 50, 00161 Rome, Italy.
Genetica. 2003 Sep;119(1):99-106. doi: 10.1023/a:1024446910161.
The Japanese rose bitterling, Rhodeus ocellatus kurumeus, and the oily bitterling, Tanakia limbata, were cytogenetically studied by silver (Ag)- and chromomycin A3 (CMA3)-staining, by C-banding and by mapping of the 18S ribosomal genes and of the (TTAGGG)n telomeric sequence. These two representative species of related genera of the subfamily Acheilognathinae show very similar chromosome complements. Nevertheless, significant differences in the chromosomal distribution of nucleolus organizer regions (NORs) and interstitial telomeric sequences were observed. Whereas R. ocellatus kurumeus shows a single NOR-bearing chromosome pair, T. limbata is characterized by a higher number of variable NORs. Multiple telomeric sequence sites were found at the pericentromeric regions of several chromosomes in the rose bitterling. No telomeric sequence sites were detected near centromeres, but they were found to be scattered along the NORs in the oily bitterling. Two karyoevolutive trends might have been identified in the subfamily.
通过银（Ag）染色、放线菌素A3（CMA3）染色、C带以及18S核糖体基因和（TTAGGG）n端粒序列定位，对日本玫瑰无须魮（Rhodeus ocellatus kurumeus）和油无须魮（Tanakia limbata）进行了细胞遗传学研究。雅罗鱼亚科相关属的这两个代表性物种显示出非常相似的染色体组。然而，观察到核仁组织区（NORs）和间质性端粒序列的染色体分布存在显著差异。日本玫瑰无须魮显示出一对带有NOR的染色体，而油无须魮的特征是可变NOR的数量较多。在玫瑰无须魮的几条染色体的着丝粒周围区域发现了多个端粒序列位点。在着丝粒附近未检测到端粒序列位点，但在油无须魮中发现它们沿着NORs分散分布。在该亚科中可能已经确定了两种核型进化趋势。
