Forster T, Roy D, Ghazal P
Scottish Centre for Genomic Technology and Informatics (GTI), The University of Edinburgh, Medical School, Little France Crescent, Edinburgh EH16 4SB, UK.
J Endocrinol. 2003 Aug;178(2):195-204. doi: 10.1677/joe.0.1780195.
Microarrays are a powerful method for the global analysis of gene or protein content and expression, opening up new horizons in molecular and physiological systems. This review focuses on the critical aspects of acquiring meaningful data for analysis following fluorescence-based target hybridisation to arrays. Although microarray technology is adaptable to the analysis of a range of biomolecules (DNA, RNA, protein, carbohydrates and lipids), the scheme presented here is applicable primarily to customised DNA arrays fabricated using long oligomer or cDNA probes. Rather than provide a comprehensive review of microarray technology and analysis techniques, both of which are large and complex areas, the aim of this paper is to provide a restricted overview, highlighting salient features to provide initial guidance in terms of pitfalls in planning and executing array projects. We outline standard operating procedures, which help streamline the analysis of microarray data resulting from a diversity of array formats and biological systems. We hope that this overview will provide practical initial guidance for those embarking on microarray studies.
微阵列是对基因或蛋白质含量及表达进行全局分析的强大方法，为分子和生理系统开辟了新视野。本综述聚焦于基于荧光的靶标与阵列杂交后获取有意义数据用于分析的关键方面。尽管微阵列技术适用于多种生物分子（DNA、RNA、蛋白质、碳水化合物和脂质）的分析，但此处介绍的方案主要适用于使用长寡聚物或cDNA探针制作的定制DNA阵列。本文的目的不是全面综述微阵列技术和分析技术（这两个领域都庞大且复杂），而是提供一个有限的概述，突出显著特征，以便在规划和执行阵列项目时针对陷阱提供初步指导。我们概述了标准操作程序，这有助于简化对来自多种阵列格式和生物系统的微阵列数据的分析。我们希望这一概述能为那些着手进行微阵列研究的人员提供实用的初步指导。
