Pitout J D D, Reisbig M D, Venter E C, Church D L, Hanson N D
Department of Pathology & Laboratory Medicine, University of Calgary, Calgary T3C 0J5, Canada.
J Clin Microbiol. 2003 Aug;41(8):3933-5. doi: 10.1128/JCM.41.8.3933-3935.2003.
Detection of extended-spectrum beta-lactamases (ESBLs) in AmpC-producing Enterobacteriaceae is problematic. A modification of the double-disk test (MDDT) has been developed for successful detection of ESBLs in gram-negative bacilli producing well-characterized beta-lactamases as well as 212 clinical isolates of Enterobacter cloacae, Enterobacter aerogenes, Serratia marcescens, and Citrobacter freundii. MDDT accurately differentiated between ESBL producers and derepressed chromosomal AmpC mutants. MDDT provides a cost-effective alternative approach for clinical microbiology laboratories for routine susceptibility testing with simultaneous detection of ESBLs in enterobacteriaceae.
在产AmpC酶的肠杆菌科细菌中检测超广谱β-内酰胺酶(ESBLs)存在问题。已开发出一种双纸片试验的改良方法(MDDT),用于成功检测产特征明确的β-内酰胺酶的革兰氏阴性杆菌以及212株阴沟肠杆菌、产气肠杆菌、粘质沙雷氏菌和弗氏柠檬酸杆菌的临床分离株中的ESBLs。MDDT能准确区分ESBLs生产者和去阻遏染色体AmpC突变体。MDDT为临床微生物实验室进行常规药敏试验并同时检测肠杆菌科细菌中的ESBLs提供了一种经济有效的替代方法。