Zarrabeitia María T, Riancho José A, Gusmão Leonor, Lareu María V, Sañudo Carolina, Amorim António, Carracedo Angel
Unidad de Medicina Legal, Facultad de Medicina, Universidad de Cantabria C/Herrera Oria, s/n, 39011, Santander, Spain.
Int J Legal Med. 2003 Oct;117(5):306-11. doi: 10.1007/s00414-003-0393-4. Epub 2003 Aug 2.
DNA typing of 8 recently described STRs on the Y chromosome was carried out by means of 2 multiplex amplification reactions for 134 unrelated males from Cantabria, a region in northern Spain. Multiplex 1 included loci DYS460 (GATA A7.1), GATA A10, GATA H4 and DYS439; multiplex 2 included DYS461 (GATA A7.2), GATA C4, DYS437 and DYS438. Haplotype diversity was found to be 99.36%, similar to that obtained with the standard 9-STR set ("minimal haplotype") of the European Y-user group (99.35%). The 13-locus haplotype resulting from the combination of the standard minimal haplotype and the 4-locus multiplex 1 showed a 99.89% diversity. Further inclusion of the 4 loci in multiplex 2 resulted in a haplotype diversity of 99.93%. The combination of the "minimal haplotype" and the multiplex 1 in the present study may be an efficient way of increasing the power of discrimination in forensic cases.
通过对来自西班牙北部坎塔布里亚地区的134名无关男性进行2次多重扩增反应,对Y染色体上最近描述的8个短串联重复序列(STR)进行了DNA分型。多重反应1包括DYS460(GATA A7.1)、GATA A10、GATA H4和DYS439位点;多重反应2包括DYS461(GATA A7.2)、GATA C4、DYS437和DYS438位点。单倍型多样性为99.36%,与欧洲Y染色体用户组的标准9个STR组合(“最小单倍型”)所获得的结果相似(99.35%)。由标准最小单倍型和4个位点的多重反应1组合产生的13个位点的单倍型显示出99.89%的多样性。进一步纳入多重反应2中的4个位点,单倍型多样性达到99.93%。本研究中“最小单倍型”与多重反应1的组合可能是提高法医案件鉴别能力的有效方法。