人类Y染色体特异性短串联重复序列的黑猩猩同源物。一项比较研究及命名建议。
Chimpanzee homologous of human Y specific STRs. A comparative study and a proposal for nomenclature.
作者信息
Gusmão Leonor, González-Neira Annabel, Alves Cíntia, Lareu Maviky, Costa Solange, Amorim António, Carracedo Angel
机构信息
Instituto de Patologia e Imunologia Molecular da Universidade do Porto (IPATIMUP), R. Dr. Roberto Frias, s/n, 4200 Porto, Portugal.
出版信息
Forensic Sci Int. 2002 Apr 18;126(2):129-36. doi: 10.1016/s0379-0738(02)00046-4.
Eleven Y specific microsatellites, previously studied in humans, were typed for fragment length and sequenced in chimpanzees (Pan troglodytes). The primers described by Ayub et al. (Nucleic Acids Res. 28, 2000, 2) for amplifying DYS434, DYS435, DYS436, DYS437, DYS438, DYS439 and those described by White et al. (Genomics, 57, 1999, 433) for GATA A10, A7.1, A7.2, C4, and H4, were used to amplify DNA samples from chimpanzees. Primers described for Y GATA A4 were found to amplify the same region as reported for DYS439. Moreover, the GATA A4 forward primer only matches the repeat flanking region in 14 of the 28bp, being responsible for a very weak amplification. Therefore, this system was not included in this study. The analysis of the repeat and sequence structure observed in chimpanzee and human Y chromosomes allowed evolutionary comparisons as well as the basis for improving Y STR nomenclature and therefore, a unified nomenclature for these novel STRs is proposed to the scientific community following ISFG recommendations.
对之前在人类中研究过的11个Y染色体特异性微卫星进行了分型，测定其片段长度，并在黑猩猩（Pan troglodytes）中进行测序。Ayub等人（《核酸研究》，28卷，2000年，第2期）描述的用于扩增DYS434、DYS435、DYS436、DYS437、DYS438、DYS439的引物，以及White等人（《基因组学》，57卷，1999年，433页）描述的用于GATA A10、A7.1、A7.2、C4和H4的引物，被用于扩增黑猩猩的DNA样本。发现用于Y GATA A4的引物扩增的区域与报道的DYS439相同。此外，GATA A4正向引物在28bp中只有14个与重复侧翼区域匹配，导致扩增非常微弱。因此，该系统未纳入本研究。对在黑猩猩和人类Y染色体中观察到的重复和序列结构进行分析，有助于进行进化比较，并为改进Y染色体短串联重复序列（Y STR）命名提供依据，因此，按照国际法医遗传学会（ISFG）的建议，向科学界提出了这些新型STR的统一命名。
Zotero 插件