Bosch Elena, Lee Andrew C, Calafell Francesc, Arroyo Eduardo, Henneman Peter, de Knijff Peter, Jobling Mark A
Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK.
Forensic Sci Int. 2002 Jan 24;125(1):42-51. doi: 10.1016/s0379-0738(01)00627-2.
Nineteen Y-specific short tandem repeat (STR) loci have been amplified in 768 samples from the Iberian Peninsula in order to evaluate their usefulness in forensic casework. Two previously published multiplex reactions by Thomas et al. [Hum. Genet. 6 (1999) 577] (MS1, modified here: DYS19, DYS388, DYS390, DYS391, DYS392 and DYS393) and by Ayub et al. [Nucl. Acids Res. 28 (2000) e8] (CTS: DYS434, DYS435, DYS436, DYS437, DYS438 and DYS439) plus a novel one reported here (EBF: DYS385, DYS389, DYS460, DYS461, DYS462 and amelogenin) have been used. DYS385, DYS439 and DYS391 were the most informative loci with allele diversities of 0.7997, 0.6683 and 0.5940, respectively. A total of 635 different haplotypes were observed, of which 573 (90.24%) were found in single individuals. The overall haplotype diversity was 0.9988 and that obtained by each multiplex system was 0.9812 for EBF, 0.9292 for MS1 and 0.9089 for CTS.
为了评估19个Y染色体特异性短串联重复序列（STR）基因座在法医案件工作中的实用性，对来自伊比利亚半岛的768份样本进行了扩增。使用了Thomas等人[《人类遗传学》6（1999）577]先前发表的两种多重反应（MS1，此处修改：DYS19、DYS388、DYS390、DYS391、DYS392和DYS393）以及Ayub等人[《核酸研究》28（2000）e8]的（CTS：DYS434、DYS435、DYS436、DYS437、DYS438和DYS439），再加上此处报道的一种新的多重反应（EBF：DYS385、DYS389、DYS460、DYS461、DYS462和牙釉蛋白基因）。DYS385、DYS439和DYS391是信息含量最高的基因座，等位基因多样性分别为0.7997、0.6683和0.5940。总共观察到635种不同的单倍型，其中573种（90.24%）仅在单个个体中出现。总体单倍型多样性为0.9988，每个多重系统获得的单倍型多样性分别为：EBF为0.9812，MS1为0.9292，CTS为0.9089。
