Chen Bin, Pei Guo-xian, Wang Ke, Tang Guang-hui
Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Jun;26(6):770-3.
To observe the long-term effect of tissue engineering-based repair of large weight-bearing bone defect in goats, and the final outcome of the scaffold material coral hydroxyapatite (CHAP) in vivo.
Fifteen Chinese goats were subjected to operations to induce a 2-cm left tibial diaphyseal defect, which was filled subsequently with CHAP and bone marrow stromal stem cells (BMSCs). The repaired defects were evaluated by ECT, X-ray and histology in the early stage and at 6, 12, 18, and 24 months postoperatively.
ECT showed good bone regeneration and revascularization within 2 months postoperatively. X-ray and histology displayed eccentric and gradual bone regeneration in the early stage, and the tissue-engineered bone graft was firmly healed with the goat tibia. X-ray and histological examination at 6, 12, 18, 24 months postoperatively revealed moulding of the new bones and medullary cavity recanalization, and the structure of CHAP disappeared and gradually integrated into the new bones.
Tissue-engineered bone is capable of total repair of large bone defect in goats by forming normal functional new bones. CHAP can be eventually degraded completely and become the component of the newly generated bones.
观察基于组织工程技术修复山羊大负重骨缺损的长期效果以及支架材料珊瑚羟基磷灰石（CHAP）在体内的最终结局。
对15只中国山羊进行手术，造成左胫骨骨干2厘米的缺损，随后用CHAP和骨髓基质干细胞（BMSCs）填充。在术后早期以及术后6、12、18和24个月，通过ECT、X射线和组织学对修复的缺损进行评估。
ECT显示术后2个月内有良好的骨再生和血管再生。X射线和组织学显示早期骨再生呈偏心性且逐渐进行，组织工程骨移植物与山羊胫骨牢固愈合。术后6、12、18、24个月的X射线和组织学检查显示新骨塑形和髓腔再通，CHAP结构消失并逐渐融入新骨。
组织工程骨能够通过形成正常功能的新骨完全修复山羊的大骨缺损。CHAP最终可完全降解并成为新生成骨的组成部分。
