紫苏中生物活性三萜的高效液相色谱分析
High-performance liquid chromatographic analysis of bioactive triterpenes in Perilla frutescens.
作者信息
Chen J H, Xia Z H, Tan R X
机构信息
State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, Nanjing University, Nanjing 210093, China.
出版信息
J Pharm Biomed Anal. 2003 Aug 21;32(6):1175-9. doi: 10.1016/s0731-7085(03)00160-2.
Perilla frutescens (L.) Britt. (Lamiaceae), a famous traditional Chinese medicine, has been used for the treatment of various diseases. To evaluate the quality of P. frutescens, a simple, rapid and accurate high-performance liquid chromatography (HPLC) method was developed for the assessment of three bioactive triterpene acids: tormentic acid (TA), oleanolic acid (OA) and ursolic acid (UA). The HPLC system used an Spherisob octadecylsilyl silica (ODS) column with acetonitrile and aqueous H(3)PO(4) as the mobile phase and detection at 206 nm. The method was precise with relative standard deviations for these three constituents that ranged between 0.6-1.5% (intraday) and 0.7-2.6% (interday). The content of these three phytochemicals in the leaves of P. frutescens growing at eight different locations of China was determined to establish the effectiveness of the method.
紫苏（唇形科）是一种著名的传统中药，已被用于治疗各种疾病。为了评估紫苏的质量，开发了一种简单、快速且准确的高效液相色谱（HPLC）方法，用于测定三种生物活性三萜酸： tormentic acid（TA）、齐墩果酸（OA）和熊果酸（UA）。HPLC系统使用Spherisob十八烷基硅烷硅胶（ODS）柱，以乙腈和磷酸水溶液作为流动相，并在206nm处进行检测。该方法精密度良好，这三种成分的相对标准偏差在0.6 - 1.5%（日内）和0.7 - 2.6%（日间）之间。测定了在中国八个不同地点生长的紫苏叶中这三种植物化学物质的含量，以确定该方法的有效性。
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