Institute of Cash Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, 050051, Hebei, China.
Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi, 445000, Hubei, China.
Sci Rep. 2020 Oct 1;10(1):16207. doi: 10.1038/s41598-020-73274-y.
Perilla frutescens (L.) is an important medicinal and edible plant in China with nutritional and medical uses. The extract from leaves of Perilla frutescens contains flavonoids and volatile oils, which are mainly used in traditional Chinese medicine. In this study, we analyzed the transcriptomic and metabolomic data of the leaves of two Perilla frutescens varieties: JIZI 1 and JIZI 2. A total of 9277 differentially expressed genes and 223 flavonoid metabolites were identified in these varieties. Chrysoeriol, apigenin, malvidin, cyanidin, kaempferol, and their derivatives were abundant in the leaves of Perilla frutescens, which were more than 70% of total flavonoid contents. A total of 77 unigenes encoding 15 enzymes were identified as candidate genes involved in flavonoid biosynthesis in the leaves of Perilla frutescens. High expression of the CHS gene enhances the accumulation of flavonoids in the leaves of Perilla frutescens. Our results provide valuable information on the flavonoid metabolites and candidate genes involved in the flavonoid biosynthesis pathways in the leaves of Perilla frutescens.
荏苒（Perilla frutescens (L.)）是中国一种重要的药用和食用植物，具有营养和药用价值。荏苒的叶子提取物含有黄酮类化合物和挥发油，主要用于中药。在这项研究中，我们分析了两种荏苒品种（JIZI1 和 JIZI2）叶子的转录组和代谢组数据。在这些品种中，共鉴定出 9277 个差异表达基因和 223 种黄酮类代谢物。在荏苒的叶子中，圣草酚、芹菜素、矢车菊素、花青素、山奈酚及其衍生物含量丰富，占总黄酮含量的 70%以上。共鉴定出 77 个编码 15 种酶的基因，这些基因被认为是参与荏苒叶子中黄酮类生物合成途径的候选基因。CHS 基因的高表达促进了荏苒叶子中黄酮类化合物的积累。我们的研究结果为荏苒叶子中黄酮类代谢物和参与黄酮类生物合成途径的候选基因提供了有价值的信息。
