用高效液相色谱法分析山芝麻中三种羽扇豆烷型三萜类化合物。

Analysis of three lupane type triterpenoids in Helicteres angustifolia by high-performance liquid chromatography.

作者信息

Chang Y S, Ku Y R, Lin J H, Lu K L, Ho L K

机构信息

Institute of Chinese Pharmaceutical Sciences, China Medical College, 91, Hsueh-Shih Road, 404, Taichung, Taiwan.

出版信息

J Pharm Biomed Anal. 2001 Dec;26(5-6):849-55. doi: 10.1016/s0731-7085(01)00465-4.

DOI:10.1016/s0731-7085(01)00465-4

PMID:11600296

Abstract

Kang-chih-ma is the dried roots and stems of Helicteres angustifolia (Sterculiaceae) and a commonly used folk herbal drug in Taiwan. It possesses antidotal, analgesic, anti-inflammatory and anti-bacterial effects and is also known as a kind of tumor inhibitory plant. To evaluate the quality of H. angustifolia, a simple, rapid and accurate high-performance liquid chromatographic (HPLC) method was developed for the assay of three lupane type triterpenes: 3beta-acetoxy-27-benzoyloxylup-20(29)-en-28-oic acid methyl ester (methyl helicterate), 3beta-acetoxy-27-benzoyloxylup-20(29)-en-28-oic acid, and 3beta-acetoxy-27-(p-hydroxyl) benzoyloxylup-20(29)-en-28-oic acid methyl ester. The present HPLC system used an Inertsil ODS-2 column by gradient elution with acetonitrile and water as the mobile phase and detected at UV 230 nm. Regression equations revealed good linear relationships (correlation coefficients: 0.9922-0.9997). The relative standard deviations of these three constituents ranged between 1.05-3.14% (intraday) and 2.12-4.38% (interday). The contents of these three constituents of the heartwood and the bark of the roots of H. angustifolia in five different samples have also been determined.

摘要

康芝麻为山芝麻（梧桐科）的干燥根及茎，是台湾常用的民间草药。它具有解毒、止痛、抗炎和抗菌作用，也被认为是一种具有肿瘤抑制作用的植物。为了评估山芝麻的质量，开发了一种简单、快速且准确的高效液相色谱（HPLC）方法，用于测定三种羽扇豆烷型三萜：3β - 乙酰氧基 - 27 - 苯甲酰氧基羽扇 - 20(29) - 烯 - 28 - 酸甲酯（山芝麻甲酯）、3β - 乙酰氧基 - 27 - 苯甲酰氧基羽扇 - 20(29) - 烯 - 28 - 酸以及3β - 乙酰氧基 - 27 - （对羟基）苯甲酰氧基羽扇 - 20(29) - 烯 - 28 - 酸甲酯。本HPLC系统采用Inertsil ODS - 2柱，以乙腈和水为流动相进行梯度洗脱，并在230 nm波长处进行紫外检测。回归方程显示出良好的线性关系（相关系数：0.9922 - 0.9997）。这三种成分的相对标准偏差在日内为1.05 - 3.14%，日间为2.12 - 4.38%。还测定了五个不同样品中山芝麻根的心材和树皮中这三种成分的含量。