Stevenson Karen A, Muralidharan G, Maya Leon, Wells Jack C, Barhen Jacob, Thundat Thomas
Center for Engineering Science Advanced Research, Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
J Nanosci Nanotechnol. 2002 Jun-Aug;2(3-4):397-404. doi: 10.1166/jnn.2002.110.
Functionalized gold nanoparticles have been covalently bound to internal, modified sites on double-stranded DNA. Gold nanoparticles coated with mercaptosuccinic acid or thioctic acid were bound to amino-modified thymine bases on double-stranded DNA. Visible absorption spectra, gel electrophoresis, and atomic force microscopy were used to analyze the products. Thiol groups were added to one end of the gold/nanoparticle product, which was then attached to a gold surface. This method has the potential to allow controlled placement of particles with subnanometer precision and to allow attachment of the product to fixed contacts for nanodevice fabrication.
