Fynewever Tricia L, Agcaoili Evelyn S, Jacobson John D, Patton William C, Chan Philip J
Department of Gynecology and Obstetrics, Loma Linda University School of Medicine, Center for Fertility and IVF, 11370 Anderson Street, Suite 3950, Loma Linda, California 92354, USA.
J Assist Reprod Genet. 2007 Feb-Mar;24(2-3):61-5. doi: 10.1007/s10815-006-9084-7. Epub 2006 Dec 29.
To develop an in vitro method for tagging embryos and to compare the development of the embryos after nanoparticles injection versus externally-applied nanoparticles derived from either polystyrene or polyacrylonitrile.
Each mouse 1-cell embryo (the selected test-model) was either: (a) injected by intracytoplasmic injection or (b) co-incubated with different nanoparticles at 37 degrees C, 5% CO2 in air. The embryos were assessed after 2 and 6 days of culture.
Embryo development was similar for externally-applied polystyrene nanoparticles and control (97.6 +/- 2.7 versus 100.0 +/- 0%) but different for polyacrylonitrile nanoparticles (90.0 +/- 2.8 %) on day 2. However, the results were similar on Day 6. Injected embryos were linked to lower percent development on Day 2. Few injected embryos reached blastocyst stage on Day 6 after a brief UV-fluorescence exposure.
Tagging embryos by external polystyrene-based nanoparticles was the better method when compared with injected nanoparticles. Larger nanoparticles in microsphere range were easier to qualitate. Inhibited hatching limited their use beyond the blastocyst stage.
开发一种体外标记胚胎的方法，并比较纳米颗粒注射与外部施加源自聚苯乙烯或聚丙烯腈的纳米颗粒后胚胎的发育情况。
将每个小鼠单细胞胚胎（所选测试模型）进行如下处理：(a) 通过胞浆内注射，或 (b) 在37℃、空气中5%二氧化碳条件下与不同纳米颗粒共同孵育。培养2天和6天后对胚胎进行评估。
在第2天，外部施加的聚苯乙烯纳米颗粒与对照组的胚胎发育相似（97.6±2.7%对100.0±0%），但聚丙烯腈纳米颗粒不同（90.0±2.8%）。然而，在第6天结果相似。注射的胚胎在第2天发育百分比更低。短暂紫外线荧光照射后，很少有注射的胚胎在第6天达到囊胚阶段。
与注射纳米颗粒相比，用基于聚苯乙烯的外部纳米颗粒标记胚胎是更好的方法。微球范围内较大的纳米颗粒更易于定性。孵化受抑制限制了它们在囊胚阶段之后的应用。
