Khan P K, Awasthy K S
Department of Zoology, Patna Women's College, 800001 Patna, India.
Food Chem Toxicol. 2003 Oct;41(10):1325-8. doi: 10.1016/s0278-6915(03)00123-6.
The cytogenetic toxicity of the leaf extract of neem was evaluated in murine germ cells. The extract was found to induce structural and numerical changes in the spermatocyte chromosomes as well as synaptic disturbances in them at their first metaphase. A significant increase in the frequency of sperms with abnormal head morphology and the decrease in mean sperm count were also observed. This spermatotoxic effect of the neem extract corroborates its germ cell mutagenicity. The possible role of azadirachtin, the most active principle present in the neem extract, in producing the observed genotoxic effect is discussed.
在小鼠生殖细胞中评估了印楝叶提取物的细胞遗传毒性。发现该提取物可诱导精母细胞染色体的结构和数量变化，以及在第一次中期时的突触紊乱。还观察到头部形态异常精子的频率显著增加以及平均精子计数减少。印楝提取物的这种精子毒性作用证实了其生殖细胞致突变性。讨论了印楝提取物中最具活性的成分印楝素在产生观察到的遗传毒性作用中可能发挥的作用。
