Heredia Norma, Aréchiga Elva, Labbé Ronald, García Santos
Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Apdo. Postal 124-F, 66451 San Nicolás N.L., Mexico.
Int Microbiol. 2003 Dec;6(4):259-61. doi: 10.1007/s10123-003-0138-8. Epub 2003 Aug 9.
Various stressors were used to induce stress proteins in Clostridium perfringens. Cultures of C. perfringens FD-1041 were subjected to cold shock (28 degrees C for 1 h), acid shock (pH 4.5 for 30 min), or heat shock (50 degrees C for 30 min). Cells were lysed and protein samples were analyzed by immunoblotting with antiserum derived from rabbits suffering from gas gangrene. Eight cold shock proteins (approximate Mr 101, 82, 70, 37, 22, 12, 10 and 6 kDa) and also eight heat shock proteins (approximate Mr 101, 82, 70, 27, 22, 16, 12 and 10 kDa) were immunoreactive with the serum. No immunoreactive proteins were detected in samples subjected to acid shock proteins and purified DnaK protein was also non-immunoreactive with the serum. These immunogenic stress proteins may be important in regulating diseases caused by C. perfringens. Such proteins could be involved in cell survival mechanisms, serve as targets during infection, or play a role in recognition of the bacteria by the host.
使用多种应激源在产气荚膜梭菌中诱导应激蛋白。产气荚膜梭菌FD - 1041培养物分别经受冷休克（28℃ 1小时）、酸休克（pH 4.5 30分钟）或热休克（50℃ 30分钟）。细胞裂解后，蛋白质样品用来自患有气性坏疽兔子的抗血清进行免疫印迹分析。八种冷休克蛋白（约101、82、70、37、22、12、10和6 kDa）以及八种热休克蛋白（约101、82、70、27、22、16、12和10 kDa）与该血清发生免疫反应。在经受酸休克的样品中未检测到免疫反应性蛋白，纯化的DnaK蛋白也与该血清无免疫反应。这些具有免疫原性的应激蛋白可能在调节产气荚膜梭菌引起的疾病中起重要作用。此类蛋白可能参与细胞存活机制，在感染期间作为靶点，或在宿主识别细菌中发挥作用。
