Johnson Nicholas, Black Colin, Smith Jemma, Un Hikmet, McElhinney Lorraine M, Aylan Orhan, Fooks Anthony R
Rabies Research and Diagnostic Group, Veterinary Laboratories Agency, Weybridge, Addlestone, Surrey, KT15 3NB, UK.
J Wildl Dis. 2003 Apr;39(2):262-70. doi: 10.7589/0090-3558-39.2.262.
Sixteen rabies isolates recently collected from mainland Turkey and two isolates held within a British archive were used to form a representative cohort from a range of vectors, and were analyzed to identify potential causes for an increase of rabies within the fox (Vulpes vulpes) population in Turkey. Each isolate was characterized by sequence analysis of the nucleoprotein gene and compared phylogenetically to the cohort, to isolates from neighboring countries and to isolates from continental Europe and Russia. From this analysis the isolates could be divided into three groups associated with geographic location. This included a western group, an eastern group, and one isolate that did not group with any other Turkish isolate. This observation was also found using the heteroduplex mobility assay as an alternative method for typing rabies virus isolates. Further comparison with isolates from neighboring countries suggests that this isolate was related to viruses present in Georgia and could represent a recent import to Turkey from that country. Within the two larger groups, sequence data were obtained from both infected dogs and foxes suggesting that there has been transmission of virus between these two species. The direction of transmission could not be identified by the phylogenetic analysis, although absence of rabies within the fox population in previous years suggests that this could represent a recent spillover from the domestic dog to the fox.
最近从土耳其大陆收集的16株狂犬病病毒分离株和保存在英国档案库中的2株分离株被用于组成一个来自一系列宿主的代表性群组，并进行分析以确定土耳其狐狸（赤狐）种群中狂犬病增加的潜在原因。对每个分离株进行核蛋白基因的序列分析并进行特征鉴定，并与该群组、邻国的分离株以及欧洲大陆和俄罗斯的分离株进行系统发育比较。通过该分析，分离株可分为与地理位置相关的三组。这包括一个西部组、一个东部组，以及一个未与任何其他土耳其分离株归为一组的分离株。使用异源双链迁移率分析作为对狂犬病病毒分离株进行分型的另一种方法也发现了这一观察结果。与邻国分离株的进一步比较表明，该分离株与格鲁吉亚存在的病毒有关，可能代表最近从该国传入土耳其。在两个较大的组内，从感染的狗和狐狸中均获得了序列数据，这表明这两个物种之间存在病毒传播。尽管前几年狐狸种群中没有狂犬病表明这可能是最近从家犬传播到家狐，但系统发育分析无法确定传播方向。
