Van Houten Clayton K, Lee Belden E, Kreeger Terry J, Williams Elizabeth S, Edwards William H, Thorne E Tom, Cook Walter E, Mills Kenneth W
Department of Veterinary Sciences, University of Wyoming, Laramie, Wyoming 82071, USA.
J Wildl Dis. 2003 Apr;39(2):316-22. doi: 10.7589/0090-3558-39.2.316.
Brucellosis caused by infection with Brucella abortus is present in some elk (Cervus elaphus nelsoni) of the Greater Yellowstone Area (parts of Wyoming, Montana, and Idaho, USA). Since 1985, the Wyoming Game and Fish Department has vaccinated elk on elk feedgrounds in northwestern Wyoming during the winter months using B. abortus strain 19 (strain 19). Analysis of this vaccination program is hampered by the inability of standard serologic tests to differentiate between strain 19 vaccinated elk and those exposed to field strain B. abortus. In 1993, a competitive enzyme-linked immunosorbent assay (cELISA) was licensed to serologically differentiate between strain 19 vaccinated cattle and cattle exposed to field strain B. abortus. Seven groups of elk sera representing various B. abortus exposure histories were used to validate the cELISA test for elk. The cELISA test differentiated strain 19 vaccinated elk from elk that were challenged with B. abortus strain 2308, a pathogenic laboratory strain. The specificity of the cELISA was 96.8% for elk vaccinated with strain 19 only and sampled between 6 mo and 2 yr post vaccination, or with no B. abortus exposure. The sensitivity of the cELISA was 100%. The cELISA test will be useful in evaluating sera collected from elk in vaccinated, brucellosis endemic herds in the Greater Yellowstone Area.
由流产布鲁氏菌感染引起的布鲁氏菌病存在于大黄石地区（美国怀俄明州、蒙大拿州和爱达荷州部分地区）的一些麋鹿（加拿大马鹿尼尔森亚种）中。自1985年以来，怀俄明州渔猎部门在冬季月份使用19号流产布鲁氏菌菌株（19号菌株）对怀俄明州西北部麋鹿觅食地的麋鹿进行疫苗接种。由于标准血清学检测无法区分接种19号菌株的麋鹿和接触野外流产布鲁氏菌菌株的麋鹿，该疫苗接种计划的分析受到阻碍。1993年，一种竞争性酶联免疫吸附测定（cELISA）获得许可，用于血清学区分接种19号菌株的牛和接触野外流产布鲁氏菌菌株的牛。七组代表不同流产布鲁氏菌接触史的麋鹿血清用于验证麋鹿的cELISA检测。cELISA检测区分了接种19号菌株的麋鹿和用致病实验室菌株2308号流产布鲁氏菌攻击的麋鹿。对于仅接种19号菌株且在接种后6个月至2年之间采样或未接触流产布鲁氏菌的麋鹿，cELISA的特异性为96.8%。cELISA的敏感性为100%。cELISA检测将有助于评估从大黄石地区接种疫苗的布鲁氏菌病流行鹿群中采集的麋鹿血清。
