Patel R, Jacobs-Lorena M
Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4955.
Dev Genet. 1992;13(4):256-63. doi: 10.1002/dvg.1020130403.
Antisense RNAs have been used for gene interference experiments in many cell types and organisms. However, relatively few experiments have been conducted with antisense genes integrated into the germ line. In Drosophila reduced ribosomal protein (r-protein) gene function has been hypothesized to result in a Minute phenotype. In this report we examine the effects of antisense r-protein 49 expression, a gene known to correspond to a Minute mutation An antisense rp49 gene driven by a strong and inducible promoter was transformed into the Drosophila germ line. Induction of this gene led to the development of flies with weak Minute phenotypes and to the transient arrest of oogenesis. Parameters that may affect the success of antisense gene inactivation are discussed.
反义RNA已被用于多种细胞类型和生物体的基因干扰实验。然而，将反义基因整合到生殖系中进行的实验相对较少。在果蝇中，核糖体蛋白（r蛋白）基因功能降低被推测会导致“分钟”表型。在本报告中，我们研究了反义r蛋白49表达的影响，该基因已知与一种“分钟”突变相对应。由强诱导型启动子驱动的反义rp49基因被转化到果蝇生殖系中。该基因的诱导导致具有弱“分钟”表型的果蝇发育，并导致卵子发生的短暂停滞。文中讨论了可能影响反义基因失活成功的参数。
