Tamate H B, Patel R C, Riedl A E, Jacobs-Lorena M
Department of Genetics, Case Western Reserve University, Cleveland, OH 44106.
Mol Gen Genet. 1990 Apr;221(2):171-5. doi: 10.1007/BF00261717.
During Drosophila early development the translation of ribosomal protein mRNAs is regulated specifically and coordinately. In this study we assayed for changes of ribosomal protein rp49 gene expression in flies transformed with extra copies of the gene. RNA blot analysis revealed that the rp49 transcript was overrepresented in most of the transformed fly lines: flies carrying three times more genes than the wild type contained up to seven times more mRNA. The abundance of other ribosomal protein mRNAs was not affected. Despite the large differences in rp49 mRNA content, the proportion of the rp49 mRNA which was associated with polysomes during oogenesis and early embryogenesis did not differ significantly from the wild type, implying that rp49 protein is overproduced in the transgenic flies. The results indicate that the basis for coordinate r-protein gene expression lies in the intrinsic properties of r-protein genes, rather than in a dynamic system that separately modulates the expression of individual genes.
在果蝇早期发育过程中，核糖体蛋白mRNA的翻译受到特异性且协同的调控。在本研究中，我们检测了用该基因的额外拷贝转化的果蝇中核糖体蛋白rp49基因表达的变化。RNA印迹分析显示，rp49转录本在大多数转化的果蝇品系中过量表达：携带比野生型多三倍基因的果蝇所含mRNA最多可达七倍。其他核糖体蛋白mRNA的丰度未受影响。尽管rp49 mRNA含量存在很大差异，但在卵子发生和早期胚胎发生过程中与多核糖体相关的rp49 mRNA比例与野生型相比无显著差异，这意味着转基因果蝇中rp49蛋白过量产生。结果表明，核糖体蛋白基因协同表达的基础在于核糖体蛋白基因的内在特性，而非在于分别调节单个基因表达的动态系统。
