Kinhult J, Egesten A, Benson M, Uddman R, Cardell L O
Laboratory for Clinical and Experimental Allergy Research, Department of Otorhinolaryngology Department of Medical Microbiology, Malmö University Hospital, Malmö, Sweden.
Clin Exp Allergy. 2003 Aug;33(8):1141-6. doi: 10.1046/j.1365-2222.2003.01682.x.
The sequence of events following the recruitment of a free-flowing neutrophil in the peripheral circulation, via adhesion, migration and release of mediators, to a neutrophil on the surface of the nasal epithelium is a co-ordinated process. Little is known about the state of neutrophil activation following this course of events.
To investigate the expression of surface activation markers on neutrophils, reflecting activation during their recruitment to the nose, and to see whether the inflammatory process during allergic rhinitis influences this process.
Nine healthy controls and 12 patients with grass pollen-induced intermittent allergic rhinitis were investigated during the peak of the pollen season. The expression of CD11b, CD66b and CD63 on the neutrophil cell surface, as a reflection of activation, was analysed using flow cytometry. Neutrophils were derived from peripheral blood and nasal lavage fluid. In addition, eosinophil cationic protein (ECP) and myeloperoxidase (MPO) as well as L-, P- and E-selectins in the nasal lavage fluid were analysed using RIA and ELISA, respectively.
A marked increase in the expression of all three CD markers on the neutrophil cell surface was noticed following migration from the bloodstream to the surface of the nasal mucosa. At the peak of the grass pollen season, the MPO levels increased, reflecting an increase in the total number of nasal fluid neutrophils. In parallel, the expression of CD11b was further augmented. The expression of the CDb11b was reduced on neutrophils remaining in the circulation. In addition, the level of L-selectin was reduced on neutrophils derived from the blood during allergic inflammation.
Neutrophils might become activated during their transfer from the blood to the surface of the nasal mucosa, but these changes may also be due to depletion of activated neutrophils in the blood via activated endothelial/epithelial adhesion and chemoattractant measures. The increased expression of surface activation markers during allergic rhinitis suggests roles for neutrophils in the inflammatory process.
在外周循环中，自由流动的中性粒细胞通过黏附、迁移和介质释放，被募集到鼻上皮表面，这一系列事件是一个协调的过程。关于这一系列事件后中性粒细胞的激活状态，人们了解甚少。
研究中性粒细胞表面激活标志物的表达，以反映其在募集至鼻腔过程中的激活情况，并观察变应性鼻炎期间的炎症过程是否会影响这一过程。
在花粉季节高峰期，对9名健康对照者和12名草花粉诱发的间歇性变应性鼻炎患者进行研究。使用流式细胞术分析中性粒细胞表面CD11b、CD66b和CD63的表达，以反映激活情况。中性粒细胞来源于外周血和鼻腔灌洗液。此外，分别使用放射免疫分析和酶联免疫吸附测定法分析鼻腔灌洗液中的嗜酸性粒细胞阳离子蛋白（ECP）、髓过氧化物酶（MPO）以及L-、P-和E-选择素。
从中性粒细胞从血流迁移至鼻黏膜表面后，观察到中性粒细胞表面所有三种CD标志物的表达均显著增加。在草花粉季节高峰期，MPO水平升高，反映鼻腔液中性粒细胞总数增加。同时，CD11b的表达进一步增强。循环中剩余中性粒细胞上CDb11b的表达降低。此外，变应性炎症期间，血液来源的中性粒细胞上L-选择素水平降低。
中性粒细胞在从血液转移至鼻黏膜表面的过程中可能被激活，但这些变化也可能是由于通过激活的内皮/上皮黏附及趋化因子作用，血液中激活的中性粒细胞耗竭所致。变应性鼻炎期间表面激活标志物表达增加，提示中性粒细胞在炎症过程中发挥作用。
