类囊体结合抗坏血酸过氧化物酶突变体表现出电子传递和光合活性受损。
Thylakoid-bound ascorbate peroxidase mutant exhibits impaired electron transport and photosynthetic activity.
作者信息
Danna Cristian H, Bartoli Carlos G, Sacco Francisco, Ingala Lorena R, Santa-María Guillermo E, Guiamet Juan J, Ugalde Rodolfo A
机构信息
Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín, Avenida General Paz entre Albarellos y Constituyentes, Instituto Nacional de Tecnología Industrial, CP 1650, San Martín, Provincia de Buenos Aires, Argentina.
出版信息
Plant Physiol. 2003 Aug;132(4):2116-25. doi: 10.1104/pp.103.021717.
Abstract
In chloroplasts, stromal and thylakoid-bound ascorbate peroxidases (tAPX) play a major role in the removal of H(2)O(2) produced during photosynthesis. Here, we report that hexaploid wheat (Triticum aestivum) expresses three homeologous tAPX genes (TaAPX-6A, TaAPX-6B, and TaAPX-6D) mapping on group-6 chromosomes. The tAPX activity of a mutant line lacking TaAPX-6B was 40% lower than that of the wild type. When grown at high-light intensity photosystem II electron transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.
摘要
在叶绿体中，基质型和类囊体结合型抗坏血酸过氧化物酶（tAPX）在清除光合作用过程中产生的H₂O₂方面发挥着主要作用。在此，我们报道六倍体小麦（Triticum aestivum）表达了三个位于6号染色体群上的同源tAPX基因（TaAPX - 6A、TaAPX - 6B和TaAPX - 6D）。缺失TaAPX - 6B的突变系的tAPX活性比野生型低40%。当在高光强度下生长时，该突变体的光合系统II电子传递、光合活性和生物量积累均显著降低，这表明tAPX活性对于光合作用至关重要。尽管tAPX活性降低，但突变体植株并未表现出氧化损伤，这可能是由于光化学活性降低所致。这可能是一种补偿机制的结果，以防止氧化损伤导致tAPX突变体植株生长下降。
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