Chu Jung Kyun, Chang Min Choo
Department of Parasitology, School of Medicine, Kyung-Hee University, Korea.
Kisaengchunghak Chapchi. 1975 Jun;13(1):53-59. doi: 10.3347/kjp.1975.13.1.53.
A Study on glutamic pyruvic and oxaloacetic transaminase of different organs(e.g intestine, seminal vesicle, reticular tissue, uterus, ovary, testes) in Ascaris lumbricoides suis have been investigated. The activity of transaminase were determined on the whole homogenates and subcellular fractions separated by differential centrifugation. The activity of glutamic pyruvic and oxaloacetic were assayed by colorimetric method of Reitman-Frankel. The results were obtained as follows: 1. About ninty percent of the glutamic pyruvic and oxaloacetic transaminase in different organs was found to be localized in the supernatant fraction with the separation of differential centrifugation. And it was found that ten percent of glutamic pyruvic and oxaloacetic transaminase exists in the mitochondrial fraction. 2. The specific activity of glutamic oxaloacetic transnaminase in different organs was relatively higher than the glutamic pyruvic transaminase activity.
对猪蛔虫不同器官（如肠道、精囊、网状组织、子宫、卵巢、睾丸）中的谷丙转氨酶和谷草转氨酶进行了研究。通过差速离心分离出全匀浆和亚细胞组分，测定转氨酶的活性。采用赖特曼 - 弗兰克尔比色法测定谷丙转氨酶和谷草转氨酶的活性。结果如下：1. 在差速离心分离时，不同器官中约90%的谷丙转氨酶和谷草转氨酶存在于上清液组分中。并且发现10%的谷丙转氨酶和谷草转氨酶存在于线粒体组分中。2. 不同器官中谷草转氨酶的比活性相对高于谷丙转氨酶的活性。
