Shang Hung-Sheng, Shih Yung-Luen, Chen Chao-Ping, Lee Mei-Hui, Lu Hsu-Feng, Chou Pei-Yi, Liao Nien-Chieh, Chen Yung-Liang, Hsueh Shu-Ching, Chung Jing-Gung
Graduate Institute of Clinical of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, R.O.C.
Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C.
In Vivo. 2018 Jul-Aug;32(4):783-790. doi: 10.21873/invivo.11308.
BACKGROUND/AIM: Laminarin, mainly found in the fronds of Laminaria, has antimicrobial characteristics and induces immune responses. However, there are no available information to show the laminarin effect on glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) levels in mice with leukemia in vivo.
Fifty normal BALB/c mice were separated randomly into five groups. Group I mice received normal diet as control. Leukemia was generated in groups II-V using WEHI-3 cells: Group II mice received normal diet as positive control; group III, IV and V mice received laminarin at 1, 2.5 and 5 mg/ml with ddHO, respectively, by oral gavage every 2 days for 14 days (total of seven times). All mice were weighed during the treatment. After treatment, mice were sacrificed, blood was collected for determination of cell markers, liver and spleen samples were weighed, and spleens were used for phagocytosis and natural killer (NK) cell activity and cell proliferation using flow cytometric assay.
Laminarin did not affect animal appearances, but increased the body weight at all doses. It reduced the weight of liver at 2.5 and 5 mg/ml and of spleen at 5 mg/ml. Laminarin increased CD3 (2.5 mg/ml) and CD19 (1 and 5 mg/ml) cell populations but reduced CD11b (5 mg/ml) cell populations, however, these did not affect Mac-3 marker level. Laminarin at 1 mg/ml increased phagocytosis by macrophages from peripheral blood mononuclear cell, but did not affect those from the peritoneal cavity. Laminarin increased NK cell cytotoxic activity at all doses and at a target ratio of 25:1 and 50:1. Laminarin did not affect B-cell proliferation, but at 5 mg/ml significantly reduced T-cell proliferation. Laminarin restored glutamate oxaloacetate transaminase (2.5 and 5 mg/ml) and glutamate pyruvate transaminase (2.5 mg/ml) levels. Based on these results, we suggest that laminarin can promote immune responses and protect against liver injury.
背景/目的：海带多糖主要存在于海带的叶片中，具有抗菌特性并能诱导免疫反应。然而，尚无关于海带多糖对体内白血病小鼠谷氨酸草酰乙酸转氨酶（GOT）和谷氨酸丙酮酸转氨酶（GPT）水平影响的相关信息。
将50只正常BALB/c小鼠随机分为五组。第一组小鼠给予正常饮食作为对照。使用WEHI-3细胞在第二至五组诱导白血病：第二组小鼠给予正常饮食作为阳性对照；第三、四、五组小鼠分别以1、2.5和5mg/ml的海带多糖与双蒸水（ddHO）混合，每2天经口灌胃一次，共14天（总计七次）。治疗期间对所有小鼠称重。治疗后，处死小鼠，采集血液用于细胞标志物测定，称量肝脏和脾脏样本重量，并使用流式细胞术分析脾脏的吞噬作用、自然杀伤（NK）细胞活性及细胞增殖情况。
海带多糖不影响动物外观，但所有剂量均使体重增加。2.5和5mg/ml剂量的海带多糖使肝脏重量减轻，5mg/ml剂量使脾脏重量减轻。海带多糖增加了CD3（2.5mg/ml）和CD19（1和5mg/ml）细胞群数量，但减少了CD11b（5mg/ml）细胞群数量，不过这些并未影响Mac-3标志物水平。1mg/ml的海带多糖增加了外周血单核细胞来源巨噬细胞的吞噬作用，但对腹腔来源巨噬细胞无影响。所有剂量的海带多糖在靶细胞比例为25:1和50:1时均增加了NK细胞的细胞毒性活性。海带多糖不影响B细胞增殖，但5mg/ml时显著降低T细胞增殖。海带多糖使谷氨酸草酰乙酸转氨酶（2.5和5mg/ml）及谷氨酸丙酮酸转氨酶（2.5mg/ml）水平恢复正常。基于这些结果，我们认为海带多糖可促进免疫反应并预防肝损伤。
