Yokoyama H, Ishii H, Moriya S, Nagata S, Watanabe T, Kamegaya K, Takahashi H, Maruyama K, Haber P, Tsuchiya M
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
J Hepatol. 1995 Feb;22(2):130-4. doi: 10.1016/0168-8278(95)80419-6.
The influence of hepatitis C virus and its subtypes on the clinical course of liver disease in alcoholics was assessed. Hepatitis C virus infection was confirmed by a reverse transcription and polymerase chain reaction method for the hepatitis C virus NS-5 region in the sera of alcoholics with various stages of histologically proven liver disease. The frequency of hepatitis C virus was significantly higher in alcoholics with chronic hepatitis (73%) than in those with liver fibrosis (18%), alcoholic hepatitis (17%), and fatty liver (0%). Hepatitis C virus subtypes, namely K1 and K2, were determined by dot-blot hybridization analysis of the polymerase chain reaction products with specific probes, and their frequencies were 68% and 32%, respectively. The proportion of patients whose serum transaminase levels returned to normal following 4 weeks of abstinence in hospital was significantly lower in alcoholics with hepatitis C virus viremia (glutamic oxaloacetic transaminase: 53.8%; glutamic pyruvic transaminase: 42.3%) than in those without viremia (glutamic oxaloacetic transaminase: 86.2%, p < 0.01; glutamic pyruvic transaminase: 89.7%, p < 0.01). When alcoholics with the K1 and K2 subtypes of hepatitis C virus were compared, normalization of transaminase levels was less frequent in alcoholics with K1 (glutamic oxaloacetic transaminase: 42.8%; glutamic pyruvic transaminase: 28.6%) than in those with K2 (glutamic oxaloacetic transaminase: 88.9%, p < 0.05; glutamic pyruvic transaminase: 77.8%, P < 0.05). These data indicate that hepatitis C virus infection is associated with a reduced rate of recovery of serum transminase levels following abstinence in subjects with alcoholic liver disease, more so in the K1 subtype than in the K2 subtype.
评估了丙型肝炎病毒及其亚型对酗酒者肝病临床病程的影响。通过逆转录和聚合酶链反应方法，对组织学确诊的不同阶段肝病的酗酒者血清中的丙型肝炎病毒NS-5区域进行检测，以确认丙型肝炎病毒感染情况。慢性肝炎酗酒者中丙型肝炎病毒的感染率（73%）显著高于肝纤维化（18%）、酒精性肝炎（17%）和脂肪肝（0%）的酗酒者。通过用特异性探针进行聚合酶链反应产物的斑点杂交分析确定丙型肝炎病毒亚型，即K1和K2，其频率分别为68%和32%。丙型肝炎病毒血症的酗酒者在住院戒酒4周后血清转氨酶水平恢复正常的患者比例（谷草转氨酶：53.8%；谷丙转氨酶：42.3%）显著低于无病毒血症者（谷草转氨酶：86.2%，p<0.01；谷丙转氨酶：89.7%，p<0.01）。比较丙型肝炎病毒K1和K2亚型的酗酒者时，K1亚型酗酒者转氨酶水平正常化的频率（谷草转氨酶：42.8%；谷丙转氨酶：28.6%）低于K2亚型酗酒者（谷草转氨酶：88.9%，p<0.05；谷丙转氨酶：77.8%，P<0.05）。这些数据表明，丙型肝炎病毒感染与酒精性肝病患者戒酒血清转氨酶水平恢复率降低有关，K1亚型比K2亚型更明显。
