Li Bu-hong, Xie Shu-sen, Lu Zu-kang
National Research Center of Engineering and Technology for Optical Instrumentation, Zhejiang University, Hangzhou 310027, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2002 Dec;22(6):902-4.
The spectral properties of new photosensitizer ZnPcS2P2, PsD-007 and HMME, as well as traditional photosensitizer HpD have been studied by comparing their spectra in physiological saline and in physiological saline with 10 percent serum. Experimental results show that the maximum absorption peaks for PsD-007, HMME and HpD in the physiological saline with 10 percent serum appear at 400 nm in the soret region, while at 670 nm for ZnPcS2P2. The fluorescence excitation spectra closely resemble the absorption spectra. When excited by the light at the wavelengths of 413 and 514.5 nm, the fluorescence emission peaks for PsD-007, HMME and HpD appear at 625 and 690 nm, respectively. The fluorescent excitation efficiency of the same photosensitizer with the same concentration excited by the light at the wavelength of 413 nm is about three fold higher than that at 514.5 nm. Furthermore, the fluorescent excitation efficiency is the highest for HMME, but is lower for HpD and lowest for PsD-007. These results are significant in the selection of photosensitizers for photodynamic diagnosis and therapy.
通过比较新型光敏剂二氢卟吩e6（ZnPcS2P2）、光动力敏剂-007（PsD-007）、血卟啉单甲醚（HMME）以及传统光敏剂血卟啉衍生物（HpD）在生理盐水和含10%血清的生理盐水中的光谱，研究了它们的光谱特性。实验结果表明，在含10%血清的生理盐水中，PsD-007、HMME和HpD在索雷特带的最大吸收峰出现在400nm处，而ZnPcS2P2的最大吸收峰出现在670nm处。荧光激发光谱与吸收光谱非常相似。当用413nm和514.5nm波长的光激发时，PsD-007、HMME和HpD的荧光发射峰分别出现在625nm和690nm处。相同浓度的同一光敏剂在413nm波长光激发下的荧光激发效率比在514.5nm波长光激发下高约三倍。此外，HMME的荧光激发效率最高，HpD较低，PsD-007最低。这些结果对光动力诊断和治疗中光敏剂的选择具有重要意义。
