Han Guangliang, Yang Xiaoguang, Piao Jianhua
Institute of Nutrition and Food Safety, Center for Disease Control and Prevention, Beijing 100050, China.
Wei Sheng Yan Jiu. 2003 May;32(3):225-7.
Isotope technique is the most accurate method to assess the bioavailability of trace elements. Due to the hazard of radioisotopes, it can't be used to such populations as infants and pregnant women. With the development of stable isotope technique, more and more researches were conducted with stable isotopes. The newly developed dual stable isotope technique was preferred because of its accuracy. While the papers published using such technique till now usually contained the estimation of the blood volume and hemoglobin incorporation. Because of such estimation, errors would be introduced into the results. To obtain the accurate result from the data available, this paper described a rigorous method to calculate the trace element bioavailability. The dual stable isotopic technique was discussed in detail in this paper. From the assumption of the method to their performance, the author described the procedure of calculation of the mineral absorption from the MS data and why. This method was described by using iron as an example, and can be used in similar studies.
同位素技术是评估微量元素生物利用度最准确的方法。由于放射性同位素的危害，它不能用于婴儿和孕妇等人群。随着稳定同位素技术的发展，越来越多的研究采用稳定同位素进行。新开发的双稳定同位素技术因其准确性而更受青睐。然而，迄今为止使用该技术发表的论文通常都包含血容量和血红蛋白掺入量的估算。由于这种估算，结果会引入误差。为了从现有数据中获得准确结果，本文描述了一种计算微量元素生物利用度的严谨方法。本文详细讨论了双稳定同位素技术。从该方法的假设到其性能，作者描述了从质谱数据计算矿物质吸收的过程及原因。该方法以铁为例进行描述，可用于类似研究。
