Dastidar S G, Chakrabarty A N
Department of Pharmaceutical Technology, Jadavpur University, Calcutta.
Indian J Med Res. 1992 Nov;95:263-9.
Gamma-irradiated splenic homogenates of armadillos infected with M. leprae proved sterile by conventional tests and media. However, on media for chemoautotrophy, these could repeatedly grow as a single type of acid-fast nocardioform bacterium like the unirradiated specimens, although with a much reduced count. In the slide culture, transition from the initial AFB/coccoid bodies, to sporulating mycelia and granules in the final stage, could be observed sequentially. The gamma-irradiated tissue specimens failed to yield any other mycobacterium/corynebacterium tested according to standard protocols.
用传统检测方法和培养基检测发现，感染麻风分枝杆菌的犰狳经伽马射线照射后的脾脏匀浆无菌。然而，在化学自养培养基上，这些匀浆能像未照射的标本一样，反复培养出单一类型的抗酸诺卡氏菌样细菌，尽管数量大幅减少。在玻片培养中，可依次观察到从最初的抗酸杆菌/球状体到最终阶段形成芽孢的菌丝体和颗粒的转变。根据标准方案，经伽马射线照射的组织标本未能培养出任何其他检测的分枝杆菌/棒状杆菌。
