Dastidar S G, Chakrabarty A N
Division of Microbiology, Jadavpur University, Calcutta.
Indian J Med Res. 1990 Mar;91:98-105.
A nocardioform bacterium was isolated from the spleen tissue of an armadillo infected with M. leprae and easily propagated in pure culture in mineral salt medium supplemented with only simple C and N sources (e.g., liquid paraffin, tetradecane, ammonium salts, urea, asparagine, gelatin, xanthin, hypoxanthin etc.). Complex organic substances, e.g., tyrosin, casein, peptone, meat extract, egg proteins, serum, blood, yeast extract as well as medium 199, did not support the growth of this organism. Microscopically, the organism consisted of acid-fast, long, slender rods which originated from long, fragmented hyphae, or sporulating mycelial tufts; it was acid-fast (at less than 4.0% H2SO4) which was pyridine-susceptible. It produced DOPA-oxidase and Catalase and was lysozyme resistant; this grew best under reduced O2 tension, at pH 7.0 to 8.0 and 28 degrees C. Serologically, it appeared to be only weakly related to the prototype human multibacillary leprosy-derived (reference) nocardioform strain, Nocardia brasiliensis and N. caviae, but was variably related to several mycobacteria strains.
从感染麻风分枝杆菌的犰狳脾脏组织中分离出一种诺卡氏菌样细菌，该细菌在仅添加简单碳源和氮源（如液体石蜡、十四烷、铵盐、尿素、天冬酰胺、明胶、黄嘌呤、次黄嘌呤等）的无机盐培养基中易于纯培养繁殖。复杂有机物质，如酪氨酸、酪蛋白、蛋白胨、肉浸液、卵蛋白、血清、血液、酵母浸膏以及培养基199，均不能支持该菌生长。显微镜下，该菌由抗酸、细长的杆菌组成，这些杆菌源自长的、断裂的菌丝或产孢菌丝束；它抗酸（在硫酸浓度低于4.0%时）且对吡啶敏感。它产生多巴氧化酶和过氧化氢酶，对溶菌酶有抗性；该菌在氧气张力降低、pH值为7.0至8.0以及28℃的条件下生长最佳。血清学上，它似乎与源自人类多菌型麻风病的原型诺卡氏菌样菌株、巴西诺卡菌和豚鼠诺卡菌仅有微弱关联，但与几种分枝杆菌菌株存在不同程度的关联。
