Henderson D J, Bennett P R, Moore G E
Institute of Obstetrics and Gynaecology, Royal Postgraduate Medical School, University of London, Queen Charlotte's and Chelsea Hospital, UK.
Hum Reprod. 1992 Nov;7(10):1474-8. doi: 10.1093/oxfordjournals.humrep.a137597.
The expression of the placental proteins human chorionic gonadotrophin alpha (HCG alpha), beta (HCG beta) and human placental lactogen (HPL) was examined in trophoblast from human normal pregnancy and early embryonic failure (EEF) using Northern blot analysis. Trophoblast from EEF expressed significantly depressed levels of HCG alpha (P < 0.05) and HCG beta (P < 0.01) when compared to normal pregnancy. Levels of expression of HPL in EEF were not significantly different to those from normal pregnancy. The gestational ages of the two groups were not significantly different. Immunocytochemistry on paraffin-fixed tissue sections supported these data and showed that mRNA levels reflected protein production of HCG within the tissue. It appears from these data that the depressed level of HCG found in the serum of women with early pregnancy failure is not solely a consequence of diminished placentation in these pregnancies, or placental necrosis, but that the genes of the alpha and beta HCG subunits are down-regulated. This study further suggests that there are two subsets of women with early pregnancy failure; those with apparently normal levels of placental proteins, and those with severely depressed levels. It would seem likely that these two groups have different aetiologies.
采用Northern印迹分析法，检测了来自正常妊娠和早期胚胎停育（EEF）的人胎盘绒毛膜促性腺激素α（HCGα）、β（HCGβ）和人胎盘催乳素（HPL）在滋养层中的表达。与正常妊娠相比，EEF的滋养层中HCGα（P < 0.05）和HCGβ（P < 0.01）的表达水平显著降低。EEF中HPL的表达水平与正常妊娠者无显著差异。两组的孕周无显著差异。石蜡包埋组织切片的免疫细胞化学结果支持了这些数据，并表明mRNA水平反映了组织内HCG的蛋白生成情况。从这些数据来看，早期妊娠失败女性血清中HCG水平降低，并非仅仅是这些妊娠中胎盘形成减少或胎盘坏死的结果，而是HCGα和β亚基的基因下调所致。本研究进一步表明，早期妊娠失败的女性存在两个亚组：胎盘蛋白水平明显正常的女性，以及胎盘蛋白水平严重降低的女性。这两组女性的病因似乎可能不同。
