Hoshina M, Boothby M, Hussa R, Pattillo R, Camel H M, Boime I
Placenta. 1985 Mar-Apr;6(2):163-72. doi: 10.1016/s0143-4004(85)80066-7.
Normal trophoblast of the human placenta elaborates at least two major protein hormones, chorionic gonadotrophin (hCG) and placental lactogen (hPL). Molar and choriocarcinoma tissues characteristically synthesize large amounts of hCG and hPL. To examine the role of trophoblast differentiation in the expression of the hCG and hPL genes, we studied the cytological distribution of their mRNAs in tissue sections of human hydatidiform mole and choriocarcinoma by in situ hybridization. Histologically, these tissues are in different stages of cellular differentiation. In normal placenta, hCG alpha/beta mRNA can be localized to some cytotrophoblasts and primarily to the syncytium, whereas hPL mRNA appears only in the syncytial layer. In hydatidiform mole, which still retains placental villous morphology, the hPL gene and hCG alpha and beta genes are expressed but are poorly localized because of the admixture of cyto- and syncytiotrophoblasts. By contrast, choriocarcinoma, which is devoid of placental villous pattern but in which the cyto- and syncytiotrophoblast-like components are distinguishable, expresses hCG alpha and beta in the syncytial-like areas but little, if any, hPL. These results suggest that a certain level of trophoblast differentiation, such as villous formation, is associated with hPL expression, while the hCG alpha gene and the hCG beta gene can be expressed in more disorganized tissues which contain cytotrophoblastic elements.
人胎盘的正常滋养层细胞可产生至少两种主要的蛋白质激素，即绒毛膜促性腺激素（hCG）和胎盘催乳素（hPL）。葡萄胎和绒毛膜癌组织通常会大量合成hCG和hPL。为了研究滋养层细胞分化在hCG和hPL基因表达中的作用，我们通过原位杂交技术研究了它们的mRNA在人葡萄胎和绒毛膜癌组织切片中的细胞学分布。从组织学角度来看，这些组织处于细胞分化的不同阶段。在正常胎盘中，hCGα/β mRNA可定位于一些细胞滋养层细胞，主要定位于合体滋养层，而hPL mRNA仅出现在合体滋养层。在仍保留胎盘绒毛形态的葡萄胎中，hPL基因以及hCGα和β基因均有表达，但由于细胞滋养层细胞和合体滋养层细胞混合在一起，其定位不佳。相比之下，绒毛膜癌没有胎盘绒毛结构，但其中可区分出细胞滋养层样成分和合体滋养层样成分，它在合体滋养层样区域表达hCGα和β，但几乎不表达hPL（如果有表达的话也极少）。这些结果表明，一定程度的滋养层细胞分化，如绒毛形成，与hPL表达相关，而hCGα基因和hCGβ基因可在含有细胞滋养层细胞成分的更无序组织中表达。
