Braun Martin, Atalick Stefan, Guldi Dirk M, Lanig Harald, Brettreich Michael, Burghardt Stephan, Hatzimarinaki Maria, Ravanelli Elena, Prato Maurizio, van Eldik Rudi, Hirsch Andreas
Institut für Organische Chemie Universität Erlangen-Nürnberg Henkestrasse 42, 91054 Erlangen, Germany.
Chemistry. 2003 Aug 18;9(16):3867-75. doi: 10.1002/chem.200204680.
Two dendritic fullerene (DF) monoadducts, 2 and 3, which can carry up to 9 and 18 negative charges, respectively, were examined with respect to electrostatic complexation with Cytochrome c (Cytc). To facilitate comprehensive photophysical investigations, the zinc analogue of Cytc (ZnCytc) was prepared according to a novel, modified procedure. The association of ZnCytc and DF, and consequential photoinduced electron transfer within ZnCytc-DF from the photoexcited protein to the fullerene, was proven by fluorescence spectroscopy and transient absorption spectroscopy. These findings were also supported by circular dichroism as well as by extensive molecular dynamics (MD) simulations.
研究了两种分别可携带多达9个和18个负电荷的树枝状富勒烯（DF）单加合物2和3与细胞色素c（Cytc）的静电络合情况。为便于进行全面的光物理研究，按照一种新颖的改进方法制备了细胞色素c的锌类似物（ZnCytc）。通过荧光光谱和瞬态吸收光谱证明了ZnCytc与DF的缔合以及由此在ZnCytc-DF内从光激发蛋白到富勒烯的光诱导电子转移。圆二色性以及广泛的分子动力学（MD）模拟也支持了这些发现。
