Donald Claire, Hamilton Leona, Doughty Michael J
Department of Vision Sciences, Glasgow-Caledonian University, Cowcaddens Road, Glasgow G4 0BA, Scotland, UK.
Optom Vis Sci. 2003 Aug;80(8):564-72. doi: 10.1097/00006324-200308000-00009.
To quantitatively assess the staining of Marx's line with lissamine green dye. METHODS Forty white (European) subjects, aged 18 to 78 years, were assessed. The subjects had no major eye disease, although some had mild to moderate blepharitis. Lissamine green dye strips were used to stain the marginal zone of the palpebral conjunctiva, and high-magnification photo slit lamp images were taken of the everted upper eyelid along with a scale rule. From prints at 1600x, the distances between the main tarsal (Meibomian) gland orifices, the width of the lissamine green-stained Marx's line, and the location of its anterior border were measured.
The group-mean distance between the main tarsal gland orifices was 0.81 +/- 0 16 mm, whereas the width of the lissamine green-stained line was 0.10 +/- 0.09 mm. In most subjects, the lissamine green-stained line was posterior to the tarsal gland orifices, and the location of the proximal (anterior) edge of this line averaged +0.13 mm from a reference line drawn through the orifices.
Lissamine green dye highlights a very distinct line of cells along the marginal zone. This line of cells is narrow and is thought to be the natural site of frictional contact between the eyelid margin and the surfaces of the bulbar conjunctiva and cornea, rather than the edge of the tear meniscus or location of the edge of the lacrimal river.
定量评估丽丝胺绿染料对马克思线的染色情况。方法：对40名年龄在18至78岁的白人（欧洲人）受试者进行评估。这些受试者没有重大眼部疾病，不过有些人患有轻度至中度睑缘炎。使用丽丝胺绿染料条对睑结膜边缘区进行染色，并在带有刻度标尺的情况下，对上睑外翻拍摄高倍照片裂隙灯图像。从1600倍的打印图像中，测量主要睑板（睑板腺）腺管开口之间的距离、丽丝胺绿染色的马克思线的宽度及其前缘的位置。
主要睑板腺腺管开口之间的组平均距离为0.81±0.16毫米，而丽丝胺绿染色线的宽度为0.10±0.09毫米。在大多数受试者中，丽丝胺绿染色线位于睑板腺腺管开口后方，这条线近端（前缘）的位置距离通过腺管开口绘制的参考线平均为+0.13毫米。
丽丝胺绿染料突出显示了沿边缘区的一条非常明显的细胞线。这条细胞线很窄，被认为是睑缘与球结膜和角膜表面之间摩擦接触的自然部位，而不是泪液弯月面的边缘或泪河边缘的位置。
