Lu Xiong, Leng Yang
Department of Mechanical Engineering, Hong Kong University of Science and Technology, Kowloon, Hong Kong.
J Biomed Mater Res A. 2003 Sep 1;66(3):677-87. doi: 10.1002/jbm.a.10022.
The effects of implant surface topography and chemistry on osteoblast behavior have been a research focus because of their potential importance in orthopedic and dental applications. This work focused on the topographic effects of hydroxyapatite (HA) and titanium (Ti) surface that had identical micropatterns to determine whether there was synergistic interaction between surface chemistry and surface topography. Surface microgrooves with six different groove widths (4, 8, 16, 24, 30, and 38 microm) and three different groove depths (2, 4, and 10 microm) were made on single crystalline silicon wafers using microfabrication techniques. Ti and HA thin films were coated on the microgrooves by radio-frequency magnetron sputtering. After that, human osteoblast-like cells were seeded and cultured on the microgrooved surfaces for up to 7 days. The cells' behavior was examined using scanning electron microscopy after cells were fixed and dehydrated. Statistical analysis was based on quantitative data of orientation angle, evaluating the contact guidance, and form index, describing cell shape or cell morphology changes. The contact guidance and cell shape changes were observed on the HA and Ti microgrooves. No difference in orientation angle between HA and Ti microgrooves was found. This might suggest that surface chemistry was not a significant influence on cell guidance. However, the form index analysis indicated an interaction between topographic effects and surface chemistry. Thus, conclusions about surface topographic effects on cell behavior drawn from one type of material cannot simply be applied to another type of material.
由于种植体表面形貌和化学性质在骨科和牙科应用中的潜在重要性，它们对成骨细胞行为的影响一直是研究重点。这项工作聚焦于具有相同微图案的羟基磷灰石（HA）和钛（Ti）表面的形貌效应，以确定表面化学性质和表面形貌之间是否存在协同相互作用。使用微加工技术在单晶硅片上制作了具有六种不同槽宽（4、8、16、24、30和38微米）和三种不同槽深（2、4和10微米）的表面微槽。通过射频磁控溅射在微槽上涂覆Ti和HA薄膜。之后，将人成骨样细胞接种在微槽表面并培养长达7天。在细胞固定和脱水后，使用扫描电子显微镜检查细胞行为。统计分析基于取向角的定量数据（评估接触导向）和形态指数（描述细胞形状或细胞形态变化）。在HA和Ti微槽上观察到了接触导向和细胞形状变化。未发现HA和Ti微槽之间的取向角存在差异。这可能表明表面化学性质对细胞导向没有显著影响。然而，形态指数分析表明形貌效应和表面化学性质之间存在相互作用。因此，从一种材料得出的关于表面形貌对细胞行为影响的结论不能简单地应用于另一种材料。
