Mello A, Hong Z, Rossi A M, Luan L, Farina M, Querido W, Eon J, Terra J, Balasundaram G, Webster T, Feinerman A, Ellis D E, Ketterson J B, Ferreira C L
Instituto Militar de Engenharia, IME, Rio de Janeiro, 22290-270, RJ, Brazil.
Biomed Mater. 2007 Jun;2(2):67-77. doi: 10.1088/1748-6041/2/2/003. Epub 2007 Mar 14.
Right angle magnetron sputtering (RAMS) was used to produce hydroxyapatite (HA) film coatings on pure titanium substrates and oriented silicon wafer (Si(0 0 1)) substrates with flat surfaces as well as engineered surfaces having different forms. Analyses using synchrotron XRD, AFM, XPS, FTIR and SEM with EDS showed that as-sputtered thin coatings consist of highly crystalline hydroxyapatite. The HA coatings induced calcium phosphate precipitation when immersed in simulated body fluid, suggesting in vivo bioactive behavior. In vitro experiments, using murine osteoblasts, showed that cells rapidly adhere, spread and proliferate over the thin coating surface, while simultaneously generating strong in-plane stresses, as observed on SEM images. Human osteoblasts were seeded at a density of 2500 cells cm(-2) on silicon and titanium HA coated substrates by RAMS. Uncoated glass was used as a reference substrate for further counting of cells. The highest proliferation of human osteoblasts was achieved on HA RAMS-coated titanium substrates. These experiments demonstrate that RAMS is a promising coating technique for biomedical applications.
采用直角磁控溅射（RAMS）在纯钛基底、具有平整表面的定向硅片（Si(0 0 1)）基底以及具有不同形状的加工表面上制备羟基磷灰石（HA）薄膜涂层。使用同步加速器X射线衍射、原子力显微镜、X射线光电子能谱、傅里叶变换红外光谱以及配备能谱仪的扫描电子显微镜进行分析，结果表明溅射态的薄涂层由高度结晶的羟基磷灰石组成。当将HA涂层浸入模拟体液中时会诱导磷酸钙沉淀，这表明其具有体内生物活性行为。使用小鼠成骨细胞进行的体外实验表明，细胞能在薄涂层表面迅速黏附、铺展并增殖，同时如扫描电子显微镜图像所示会产生强烈的面内应力。通过RAMS以2500个细胞/cm²的密度将人成骨细胞接种在硅和钛HA涂层基底上。未涂层的玻璃用作进一步细胞计数的参考基底。在HA RAMS涂层钛基底上实现了人成骨细胞的最高增殖。这些实验表明，RAMS是一种用于生物医学应用的有前景的涂层技术。
