胃泌素对大鼠肠上皮细胞体外分化的影响。
Effect of gastrin on differentiation of rat intestinal epithelial cells in vitro.
作者信息
Wang Zhou, Chen Wei-Wen, Li Ru-Liu, Wen Bin, Sun Jing-Bo
机构信息
Piwei Institute, Guangzhou University of TCM, Guangzhou, 510405, Guangdong Province, China.
出版信息
World J Gastroenterol. 2003 Aug;9(8):1786-90. doi: 10.3748/wjg.v9.i8.1786.
AIM
To investigate the effect of gastrin on differentiation of IEC-6 cell line in vitro.
METHODS
IEC-6 cells were incubated with gastrin. On day 7 after treatment, cell morphology was examined by light microscope, and on day 20, the cellular ultrastructures were examined by electron microscope. After exposure to gastrin for 6 hours, villin mRNA was analyzed by reverse transcription-polymerase chain reaction, and on day 7, the expression of villin was examined by immunocytochemical analysis with laser confocal microscope.
RESULTS
After exposure to gastrin, IEC-6 cells showed differentiated phenotypes as villas enterocytes and contained an abundance of plasma, small nuclei with nucleoli, and were arranged regularly. There were numerous microvilli around edge of the cells, and several cells showed columnar structures. Villin mRNA expression in cytoplasm was increased in comparison with control.
CONCLUSION
Differentiated characteristics of villus enterocytes and phenotypic changes of rat intestinal epithelial cells (IEC-6) are induced by gastrin, and the effects of gastrin are correlated to increased villin expression.
目的
研究胃泌素对体外IEC-6细胞系分化的影响。
方法
将IEC-6细胞与胃泌素一起孵育。处理后第7天，用光镜检查细胞形态，第20天，用电子显微镜检查细胞超微结构。胃泌素作用6小时后，通过逆转录-聚合酶链反应分析绒毛蛋白mRNA，第7天，用激光共聚焦显微镜通过免疫细胞化学分析检测绒毛蛋白的表达。
结果
暴露于胃泌素后，IEC-6细胞呈现出分化为绒毛肠上皮细胞的表型，含有丰富的细胞质、带核仁的小细胞核，且排列规则。细胞边缘周围有许多微绒毛，一些细胞呈现柱状结构。与对照组相比，细胞质中绒毛蛋白mRNA表达增加。
结论
胃泌素可诱导绒毛肠上皮细胞的分化特征及大鼠肠上皮细胞（IEC-6）的表型变化，胃泌素的作用与绒毛蛋白表达增加相关。
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