人肝细胞癌中I类主要组织相容性复合体分子、热休克蛋白70及抗原加工相关转运体的表达
Expression of class I MHC molecule, HSP70 and TAP in human hepatocellular carcinoma.
作者信息
Deng Xiao-Ling, Chen Wei, Cai Mei-Ying, Wei Da-Peng
机构信息
Immunology Department, West China Medical Center of Sichuan University, Chengdu 610044, Sichuan Province, China.
出版信息
World J Gastroenterol. 2003 Aug;9(8):1853-5. doi: 10.3748/wjg.v9.i8.1853.
Abstract
AIM
To demonstrate whether class I MHC molecule, transporter associated with antigen processing (TAP), and heat-shock proteion70 (HSP70) expressed in liver cancer cells before the design and construction of CTL vaccine against hepatocellular carcinoma (HCC).
METHODS
We studied 30 HCC specimens by labeled streptavidin biotin (LSAB) method of immunohistochemistry.
RESULTS
The results showed that the majority of HCC cells investigated naturally expressed class I MHC and TAP, which were different from other tumor cells. Furthermore, we found that HSP70 expressed not only in cellular cytoplasm, but also on the cell surface in HCC.
CONCLUSION
Our findings indicate that our understanding about immune escape mechanisms employed by HCC cells may be further improved. It is important to design and construct CTL vaccine against HCC.
摘要
目的
在设计和构建抗肝细胞癌（HCC）的细胞毒性T淋巴细胞（CTL）疫苗之前，证明I类主要组织相容性复合体分子、抗原加工相关转运体（TAP）和热休克蛋白70（HSP70）是否在肝癌细胞中表达。
方法
我们采用免疫组织化学的标记链霉亲和素生物素（LSAB）法研究了30例肝癌标本。
结果
结果显示，大多数被研究的肝癌细胞天然表达I类主要组织相容性复合体和TAP，这与其他肿瘤细胞不同。此外，我们发现HSP70不仅在肝癌细胞的细胞质中表达，也在细胞表面表达。
结论
我们的研究结果表明，我们对肝癌细胞所采用的免疫逃逸机制的理解可能会进一步提高。设计和构建抗肝癌的CTL疫苗很重要。
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