Sjöstedt A, Kuoppa K, Johansson T, Sandström G
Department of Microbiology, National Defence Research Establishment, Umeå, Sweden.
Microb Pathog. 1992 Sep;13(3):243-9. doi: 10.1016/0882-4010(92)90025-j.
A T-cell-stimulating 17 kDa protein of the vaccine strain Francisella tularensis LVS has previously been cloned, sequenced and shown to be a lipoprotein. In the present study, it was investigated whether the protein, denoted TUL4, and its gene are present in various strains of the genus Francisella. By Western blot analysis, it was demonstrated that a TUL4-specific monoclonal antibody bound to a protein present in each of the Francisella strains. The immunoreactive proteins had an M(r) of 17 kDa in all F. tularensis strains and in the strain Francisella novicida, whereas the M(r) in strains of Francisella philomiragia was 20 kDa. When genomic preparations were probed with a radioactive DNA fragment of F. tularensis LVS encoding TUL4, hybridization was demonstrated in all strains of Francisella, although the F. philomiragia strains did not hybridize under conditions of high stringency. The hybridizing chromosomal DNA fragment of the F. philomiragia strains was larger than that of the other Francisella strains. No hybridization or Western blot reactivity was seen when various other Gram-negative and Gram-positive bacteria were probed. In summary, the 17 kDa lipoprotein of F. tularensis LVS appears to be Francisella-specific and present in the species F. tularensis and F. novicida, whereas an immunologically related protein is present in F. philomiragia.
疫苗株土拉弗朗西斯菌LVS的一种刺激T细胞的17 kDa蛋白此前已被克隆、测序，并显示为一种脂蛋白。在本研究中，对该名为TUL4的蛋白及其基因是否存在于弗朗西斯菌属的各种菌株中进行了研究。通过蛋白质印迹分析表明，一种TUL4特异性单克隆抗体与存在于每种弗朗西斯菌菌株中的一种蛋白结合。在所有土拉弗朗西斯菌菌株和新凶手弗朗西斯菌菌株中，免疫反应性蛋白的相对分子质量为17 kDa，而在嗜肺弗朗西斯菌菌株中，其相对分子质量为20 kDa。当用编码TUL4的土拉弗朗西斯菌LVS放射性DNA片段探测基因组制剂时，在所有弗朗西斯菌菌株中均显示出杂交，尽管嗜肺弗朗西斯菌菌株在高严格条件下不杂交。嗜肺弗朗西斯菌菌株的杂交染色体DNA片段比其他弗朗西斯菌菌株的大。当探测各种其他革兰氏阴性菌和革兰氏阳性菌时，未观察到杂交或蛋白质印迹反应性。总之，土拉弗朗西斯菌LVS的17 kDa脂蛋白似乎是弗朗西斯菌属特异性的，存在于土拉弗朗西斯菌和新凶手弗朗西斯菌中，而在嗜肺弗朗西斯菌中存在一种免疫相关蛋白。
