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对镰刀菌酸、9,10-脱氢镰刀菌酸及其甲酯（杂草致病镰刀菌属物种的有毒代谢产物）的高效液相色谱分析。

HPLC analysis of fusaric acid, 9,10-dehydrofusaric acid and their methyl esters, toxic metabolites from weed pathogenic Fusarium species.

作者信息

Amalfitano Carmine, Pengue Raffaele, Andolfi Anna, Vurro Maurizio, Zonno Maria C, Evidente Antonio

机构信息

Dipartimento di Scienze Chimico-Agrarie, Università di Napoli Federico II, Via Università 100, 80055 Portici, Italy.

出版信息

Phytochem Anal. 2002 Sep-Oct;13(5):277-82. doi: 10.1002/pca.648.

DOI:10.1002/pca.648
PMID:12918873
Abstract

A simple and rapid HPLC method, using a high-density C18 column, has been developed for the quantitative analysis of fusaric and dehydrofusaric acids and their methyl esters in the methanol extract of lyophilised culture filtrates of species of Fusarium. The method has been used to determine the content of these metabolites in two strains of Fusarium oxysporum and in strains of F. nygamai and F. udum. Fusaric acid has been isolated and identified from a strain of F. udum for the first time.

摘要

已开发出一种使用高密度C18柱的简单快速高效液相色谱法，用于定量分析镰刀菌冻干培养滤液甲醇提取物中的镰刀菌酸、脱氢镰刀菌酸及其甲酯。该方法已用于测定尖孢镰刀菌的两个菌株以及尼日利亚镰刀菌和乌杜姆镰刀菌菌株中这些代谢物的含量。首次从乌杜姆镰刀菌菌株中分离并鉴定出镰刀菌酸。

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