Cosnier Serge
Laboratoire d'Electrochimie Organique et de Photochimie Redox, UMR CNRS 5630, Institut de Chimie Moléculaire de Grenoble, FR CNRS 2607, Université Joseph Fourier, BP 53, 38041, Grenoble cedex 9, France.
Anal Bioanal Chem. 2003 Oct;377(3):507-20. doi: 10.1007/s00216-003-2131-7. Epub 2003 Aug 12.
Electropolymerized films have received considerable attention in the development of biosensors and biochips, and are advancing rapidly. This paper reviews recent advances and scientific progress in electrochemical immobilization procedures for biological macromolecules on electrodes via electrogenerated polymer films. Biomolecule immobilization is classified as covalent linkage, attachment by affinity interactions, and physical entrapment. The last approach entails the use of conducting and non-conducting films, composite polymer films, and templates for the electropolymerization process. Some advances in the electrochemical transduction of biological events (enzymatic reaction, immunoreaction, or oligonucleotide hybridization) involving the redox properties or the conductivity of electropolymerized films are also presented.
电聚合膜在生物传感器和生物芯片的发展中受到了广泛关注，并且发展迅速。本文综述了通过电生成聚合物膜将生物大分子电化学固定在电极上的最新进展和科学成果。生物分子固定化可分为共价连接、通过亲和相互作用附着以及物理包埋。最后一种方法涉及在电聚合过程中使用导电和非导电膜、复合聚合物膜以及模板。文中还介绍了涉及电聚合膜的氧化还原特性或导电性的生物事件（酶反应、免疫反应或寡核苷酸杂交）的电化学传感方面的一些进展。
