Cosnier S
Laboratoire d'Electrochimie Organique et de Photochimie Rédox, UMR CNRS 5630, Université Joseph Fourier Grenoble 1, BP 53, 38041 Grenoble, France.
Appl Biochem Biotechnol. 2000 Nov-Dec;89(2-3):127-38. doi: 10.1385/abab:89:2-3:127.
The concept and potentialities of electrochemical procedures of biomolecule immobilization are described. The entrapment of biomolecules within electropolymerized films consists of the application of an appropriate potential to an electrode soaked in an aqueous solution containing monomer and biomolecules. This method of biosensor construction is compared with a two-step procedure based on the adsorption of an aqueous amphiphilic pyrrole monomer-biomolecule mixture on an electrode followed by the electropolymerization of the adsorbed monomers. Another approach is based on the electrogeneration of polymer films functionalized by specific groups allowing subsequently the attachment of biomolecules. The immobilization of biomolecules on these films by covalent binding or noncovalent interactions is described.
描述了生物分子固定化电化学方法的概念和潜力。生物分子在电聚合膜中的截留包括对浸泡在含有单体和生物分子的水溶液中的电极施加适当的电势。将这种构建生物传感器的方法与基于两亲性吡咯单体 - 生物分子混合物在电极上吸附然后吸附单体进行电聚合的两步法进行了比较。另一种方法基于由特定基团功能化的聚合物膜的电生成，随后允许生物分子附着。描述了通过共价结合或非共价相互作用将生物分子固定在这些膜上的方法。
