Yamane Hisayo, Ushijima Koichiro, Sassa Hidenori, Tao Ryutaro
Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan.
Theor Appl Genet. 2003 Nov;107(8):1357-61. doi: 10.1007/s00122-003-1389-7. Epub 2003 Aug 15.
Japanese apricot ( Prunus mume) exhibits the S-RNase-based gametophytic self-incompatibility system as do other self-incompatible Prunus species. This report identifies the S haplotype-specific F-box protein gene ( SFB), a candidate gene for pollen- S, of Japanese apricot, which leads to the development of a molecular typing system for S-haplotype in this fruit species. Both 5'- and 3'-RACE (rapid amplification of cDNA ends) were performed with SFB gene-specific oligonucleotide primers to clone Pm-SFB(1) and Pm-SFB(7) of 'Nanko ( S(1) S(7))'. As in the case of SFB of other Prunus species, Pm-SFB(1) and Pm-SFB(7) showed a high level of S-haplotype-specific sequence polymorphism and their expression was specific to pollen. Genomic DNA-blot analyses of 11 Japanese apricot cultivars with the Pm-SFB probes under low stringency conditions yielded RFLP bands specific to the S(1)- to S(8)-haplotypes as well as a self-compatible S(f)-haplotype. A practical usage of SFB as a molecular marker for S-haplotypes and self-compatibility in Japanese apricot is discussed.
与其他自交不亲和的李属物种一样，梅（Prunus mume）表现出基于S-RNase的配子体自交不亲和系统。本报告鉴定了梅的S单倍型特异性F-box蛋白基因（SFB），它是花粉S的候选基因，这有助于开发该水果物种S单倍型的分子分型系统。使用SFB基因特异性寡核苷酸引物进行5'-和3'-RACE（cDNA末端快速扩增），以克隆‘南光（S(1)S(7)）’的Pm-SFB(1)和Pm-SFB(7)。与其他李属物种的SFB情况一样，Pm-SFB(1)和Pm-SFB(7)表现出高水平的S单倍型特异性序列多态性，并且它们的表达对花粉具有特异性。在低严谨条件下，用Pm-SFB探针对11个梅品种进行基因组DNA印迹分析，产生了S(1)至S(8)单倍型以及自交亲和S(f)单倍型特异性的RFLP条带。本文讨论了SFB作为梅S单倍型和自交亲和性分子标记的实际应用。
