Dudriková E, Lenhardt L, Porácová J
Univerzita veterinárskeho lekárstva, Kosice.
Vet Med (Praha). 1992 Nov;37(11):613-22.
Fifteen Slovak Merino sheep were included in the experiment. The animals weighing 21-28 kg were divided into three groups per five animals. In a six-week feeding experiment the animals of group I were given 50 mg supermethrin per kg live weight per day while those of group II received 200, and from week four of the experiment 300 mg supermethrin per kg live weight per day. During the experiment changes of aspartate aminotransferase (EC 2.6.1.1), alanine aminotransferase (EC 2.6.1.2), acetylcholine esterase (EC 3.1.1.7), urea und creatinine levels in blood serum were observed. Six weeks after supermethrin treatment the sheep were slaughtered and histochemical evaluation of alkaline phosphatase (EC 3.1.3.2), acid phosphatase (EC 3.1.3.1) and non-specific esterase (EC 3.1.1.1) was carried out in liver, kidney, duodenum, jejunum and ileum. In the course of the experiment changes of the enzymatic activities of aspartate aminotransferase observed in both experimental groups of sheep were similar to those seen in the control group of animals (Tab. I). As compared to the starting values, no significant changes in the activity of alanine aminotransferase were observed in group II of the experiment and in the controls. However, a significantly decreased alanine aminotransferase activity could be seen in the blood serum of sheep of group I (Tab. II). In both experimental groups of animals no significant changes in the acetylcholine esterase could be seen (Tab. III). As compared to the starting values, no significant changes were observed in creatinine levels of the control and the 1st experimental group of sheep (Tab. IV). In the sheep of the 2nd group a temporary significant decrease (p < 0.05) in creatinine levels was seen. The dynamics of urea levels was similar to starting values in all animals throughout the experiment Tab. V). In the control group of animals (Fig. 1) the high density of reaction product of alkaline phosphatase was determined in the microvilli of enterocytes of the small intestine. In the small intestine of the animals of both experimental groups, the activity of this enzyme was shown to be located in the same zone (Fig. 2). In all experimental animals in the parenchyma of the liver and kidney no significant changes could be observed. In both experimental and control animals the high activity of acid phosphatase was demonstrated to be located especially in the cytoplasma of enterocytes. The activity of non-specific esterase was located in the cytoplasma of enterocytes of the small intestine, in the intestinal crypts its activity was slight up to high.(ABSTRACT TRUNCATED AT 400 WORDS)
15只斯洛伐克美利奴羊被纳入实验。体重21 - 28千克的动物被分成三组，每组五只。在为期六周的饲养实验中，第一组动物每天每千克活重给予50毫克高效氯氰菊酯，而第二组动物每天每千克活重给予200毫克，从实验第四周起给予300毫克高效氯氰菊酯。在实验期间，观察了血清中天冬氨酸转氨酶（EC 2.6.1.1）、丙氨酸转氨酶（EC 2.6.1.2）、乙酰胆碱酯酶（EC 3.1.1.7）、尿素和肌酐水平的变化。高效氯氰菊酯处理六周后，宰杀绵羊，并对肝脏、肾脏、十二指肠、空肠和回肠中的碱性磷酸酶（EC 3.1.3.2）、酸性磷酸酶（EC 3.1.3.1）和非特异性酯酶（EC 3.1.1.1）进行组织化学评估。在实验过程中，两个实验组绵羊中天冬氨酸转氨酶的酶活性变化与对照组动物相似（表I）。与初始值相比，实验第二组和对照组中丙氨酸转氨酶活性未观察到显著变化。然而，第一组绵羊血清中丙氨酸转氨酶活性显著降低（表II）。两个实验组动物中乙酰胆碱酯酶均未观察到显著变化（表III）。与初始值相比，对照组和第一实验组绵羊的肌酐水平未观察到显著变化（表IV）。第二组绵羊的肌酐水平出现暂时显著下降（p < 0.05）。在整个实验过程中，所有动物的尿素水平动态变化与初始值相似（表V）。在对照组动物（图1）中，小肠肠上皮细胞微绒毛中碱性磷酸酶反应产物密度较高。在两个实验组动物的小肠中，该酶的活性显示位于同一区域（图2）。在所有实验动物的肝脏和肾脏实质中未观察到显著变化。在实验动物和对照动物中，酸性磷酸酶的高活性均显示尤其位于肠上皮细胞的细胞质中。非特异性酯酶的活性位于小肠肠上皮细胞的细胞质中，在肠隐窝中其活性从轻微到较高。（摘要截断于400字）
